蜡状芽孢杆菌菌株SMC的间甲酚降解特性及动力学  

Characteristics and kinetics study of m-cresol biodegradation by Bacillus cereus strain SMC

作　　者：于晨阳[1] 毛缜[1] 

机构地区：[1]中国矿业大学(徐州)环境与测绘学院,江苏徐州221116

出　　处：《化工进展》2015年第5期1453-1458,共6页Chemical Industry and Engineering Progress

摘　　要：针对高浓度间甲酚废水难降解的问题,通过筛选获得一株高效降解间甲酚的菌株,并进一步探究其对间甲酚的降解特性和降解动力学。本文从某焦化厂活性污泥中经过驯化、筛选分离得到一株以间甲酚为唯一碳源的高效降解菌株,对其进行16S r DNA分子鉴定;通过测定培养液中间甲酚的剩余浓度,考察在菌株对间甲酚的最佳降解条件;并采用Haldane模型来模拟菌株降解间甲酚的动力学行为。将分离得到的菌株命名为SMC,初步鉴定为Bacillus cereus;在p H值为7.5、温度为30℃、摇床转速为150r/min的最佳培养条件下,菌株SMC在48h内对浓度1600mg/L的间甲酚降解率达97.81%,对间甲酚最大耐受能力为1900mg/L;经过Haldane模型拟合后的动力学参数为μmax=0.01252h-1,KS=34.58mg/L,Ki=479.5mg/L(R2=0.932)。菌株SMC对间甲酚有较强的降解能力,在高效处理含酚废水方面具有一定的应用前景。A bacterial strain SMC was isolated from the aerobic sludge of coking wastewater to resolve the problem of difficulty in treating effluent with high concentration of m-cresol. The characteristics and kinetics of degradation of m-cresol by the strain was investigated with m-cresol as sole source of carbon in the cultural medium. After isolation and screening,16 S r DNA gene was analyzed to identify the taxonomic position of the strain. Its degradation capability under optimal growth conditions was studied by testing the concentration of m-cresol in residue medium. Haldane type substrate inhibit model was used to simulate cell growth on m-cresol. The isolated strain named as SMC belonged to Bacillus cereus. Under the optimal conditions of p H 7.5、30℃,150r/min of shaking rate,m-cresol with 1600mg/L initial concentration would be degraded 97.81% in 48 h and the highest tolerance to m-cresol was 1900mg/L. The kinetic parameters were：μmax=0.01252h-1,KS=34.58mg/L,Ki=479.5mg/L（R2 = 0.932）. SMC could degrade m-cresol effectively,with good application potential for wastewater containing high concentration of m-cresol.

关 键 词：间甲酚 生物降解 动力学模型 废水处理 

分 类 号：X172[环境科学与工程—环境科学] TQ085[化学工程]