猫泛白细胞减少症病毒VP2基因快速检测方法的建立与应用  

DEVELOPMENT AND APPLICATION OF PCR ASSAY FOR DETECTION OF FELINE PANLEUKOPENIA VIRUS

作　　者：邱杰[1,2] 温肖会[2] 翟少伦[2] 吕殿红[2] 宁章勇[1] 魏文康[2] 

机构地区：[1]华南农业大学兽医学院,广州510642 [2]广东省农业科学院动物卫生研究所,广州510640

出　　处：《中国动物传染病学报》2015年第2期21-25,共5页Chinese Journal of Animal Infectious Diseases

基　　金：省部产学研合作科技创新平台项目(2012B090600048);广州市科普项目(2013KP093;2013KP072);广州市动物疫病诊断行业工程技术中心(穗科信字[2012]224-26号);广东省农村信息直通车工程(2012A020601010);广东省科技计划项目(2011B020306001)

摘　　要：为了调查猫泛白细胞减少症病毒(Feline panleukopenia virus,FPV)在猫群中的流行情况,根据Gen Bank已登录的FPV VP2基因序列设计PCR引物,建立了FPV PCR快速检测方法,并对3004份猫样品进行了检测。结果显示,该方法扩增的VP2基因片段为468 bp左右,检出的最小基因组DNA浓度为6.19×10-8μg/μL,检出DNA浓度低于1 fg/μL,从3004份样品中检测出13份猫泛白细胞减少症病毒核酸阳性样品。本研究针对FPV VP2基因建立的PCR快速检测方法可作为FPV临床早期诊断及快速筛查手段。To investigate the prevalence of Feline panleukopenia virus in cats, PCR method was developed using primers designed from the sequence of VP2 gene of Feline panleukopenia virus in GenBank. The amplified VP2 gene fragment was 468 bp in length. The minimal DNA detection concentration was 6.19 ×10^-8 μg/μL, which was below 1 fg/μL. This PCR method was used to test 3004 samples. Total 13 samples were confirmed positive for Feline panleukopenia virus. Therefore, the PCR method developed here is suitable for early diagnosis and epidemiological investigation of Feline panleukopenia virus in pets and wild animals.

关 键 词：猫泛白细胞减少症病毒 VP2基因 PCR 

分 类 号：S852.659.2[农业科学—基础兽医学]