姜黄素调控 Bcl －2和 Bax 蛋白表达减轻胃缺血／再灌注损伤  

Curcumin relieves gastric ischemia/reperfusion injury through regulating the expression of Bcl -2 and Bax

作　　者：孙露[1] 徐瑞宁 冷文淑[1] 刘磊[2] 乔伟丽[2] 闫长栋[2] 

机构地区：[1]徐州医学院临床医学系2010级,江苏徐州221004 [2]徐州医学院生理学教研室

出　　处：《徐州医学院学报》2015年第3期162-165,共4页Acta Academiae Medicinae Xuzhou

基　　金：江苏省大学生实践创新训练计划项目(2012JSSPITP1848)；江苏省教育厅自然科学基金

摘　　要：目的：观察姜黄素预处理对大鼠胃缺血／再灌注（gastric ischemia －reperfusion，GI／R）损伤的保护作用。方法采用夹闭大鼠腹腔动脉30 min 再灌注1 h 建立 GI／R 损伤模型。应用计算机软件分析计算 GI／R 引起的胃黏膜损伤面积，根据 whtie 评分法计算胃黏膜损伤指数，用免疫组化法与图像分析技术检测胃黏膜组织 B 细胞淋巴瘤因子2（Bcl －2）与 Bcl －2相关 X 蛋白（Bax）表达量。结果与对照组相比，GI／R 组胃黏膜损伤面积和损伤指数明显增加（P ＜0．05，P ＜0．01）；姜黄素预处理能够有效减轻 GI／R 引起的胃黏膜损伤，随着姜黄素浓度的增加，GI／R 引起的胃黏膜损伤面积和损伤指数显著减少，并具有浓度依赖性（P ＜0．05，P ＜0．01）。同时，姜黄素预处理能够降低 GI／R 引起的胃黏膜组织 Bax 蛋白表达，增加胃黏膜组织 Bcl －2蛋白表达，且随着姜黄素浓度的增加，其差异具有浓度依赖性（P ＜0．05，P ＜0．01）。结论姜黄素预处理通过增加抗凋亡蛋白 Bcl －2、减少促凋亡蛋白 Bax 的表达有效抑制 GI／R 引起的胃黏膜损伤，对胃黏膜起到一定程度的保护作用，对临床缺血／再灌注引起的器官损伤具有潜在的治疗作用。Objective To investigate the protective effects of curcumin pretreatment on the gastric ischemia/reperfu-sion （GI/R） injury in rats.Methods A rat model of GI/R injury was established through occlusion of the celiac artery in rats for 30 min followed by reperfusion for 60 min.Then the area of damaged gastric mucosa was calculated by software, while the index of gastric mucosal damages was assessed according to the white grading method.The amounts of Bcl -2 and Bax in the tissues of gastric mucosa were detected using immunohistochemical methods and image analysis. Results Compared with the control, rats in the GI/R group presented damages over remarkably larger areas and higher damage indices （P 〈0.05 or P 〈0.01）.Curcumin pretreatment could relieve the gastric mucosa injury caused by GI/R. The area of gastric mucosal damages and the damage index became significantly lowered with the rising of the concentrations of curcumin （P 〈0.05 or P 〈0.01）.Meanwhile, curcumin pretreatment could weaken the amounts of Bax but enhance the level of Bcl -2 in the gastric mucosal tissues after GI/R in a dose -dependent manner （P 〈0.05 or P 〈0.01）.Conclusion Curcumin pretreatment can protect the gastric mucosa and attenuate its damages caused by GI/R through enhancing Bcl -2 but weakening Bax protein expression.This finding indicates the potential role of curcumin as a protective agent against organ injuries caused by ischemia/reperfusion.

关 键 词：姜黄素 胃缺血/再灌注 预处理 凋亡 

分 类 号：R364.12[医药卫生—病理学]