咽炎消合剂对慢性咽炎大鼠咽黏膜修复作用的研究  

Study on the repairment of Yanyanxiao mixture in pharyngeal mucosa of rats with chronic pharyngitis

作　　者：王艺[1] 张玲[1] 周家璇[1] 

机构地区：[1]云南中医学院,云南昆明650021

出　　处：《现代中西医结合杂志》2015年第15期1600-1602,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：云南中医学院科学研究基金项目(xk201308)

摘　　要：目的研究咽炎消合剂对慢性咽炎大鼠黏膜修复的作用及其机制。方法将48只Wistar大鼠随机分为咽炎消合剂低剂量组、咽炎消合剂高剂量组、阳性药物组、抗生素组、模型组、空白对照组,每组8只。除空白对照组外,其余组采用5%氨水喷咽部制作慢性咽炎模型。造模成功后,咽炎消合剂低剂量和高剂量组分别给予咽炎消合剂1 m L/d和2m L/d灌胃,阳性药物组给予慢咽舒柠片溶解后1 m L/d灌胃,抗生素组给予阿莫西林胶囊溶解后1 m L/d灌胃。20 d后取各组咽部黏膜及其下组织进行病理切片观察。结果治疗10 d后,咽炎消合剂高、低剂量组及阳性药物组动物的一般状态好转,以咽炎消合剂高、低剂量组更明显;咽炎消合剂低、高剂量组局部症状明显好转,摄食率降低等症状也随之消失,而阳性药物组的摄食率降低、骚动不安症状改善不明显;额镜观察其咽部组织基本接近正常,部分动物虽还有轻度的慢性充血,但其咽部黏膜颜色较润泽。给药20 d后,咽炎消合剂低剂量和高剂量组咽黏膜组织病理表现接近空白对照组,咽炎消合剂高剂量组黏膜上皮增生不明显,无炎细胞浸润,固有膜改变不大,肌层纤维近于排列规则,接近于空白对照组的黏膜形态。而抗生素组病理改变不大与模型组组织变化相近。结论咽炎消合剂通过抗炎、减轻炎性渗出、抑制组织增生等多环节发挥作用,疗效显著,证明了慢性咽炎脾胃学说理论的正确性。Objective It is to observe the repairment effect of Yanyanxiao mixture（ YYX） in pharyngeal mucosa of rats with chronic pharyngitis and its mechanism. Methods 48 Wistar rats were randomly divided into YYX low-dose group,YYX high-dose group,positive drug group（ Manyanshuning）,antibiotic group（ amoxicillin）,model group and black control group,8 cases in each. Except to the blank control group,all the other groups were received 5% ammonia for spraying in pharyngeal. After successful？ animal model establishment,the rats were fed with YYX 1 m L / d and 2 m L / d orally in YYX low-dose group and YYX high-dose groups,and was given tablets dissolve Manyanshuning 1m L / d orally in the positive drug group,and was given amoxicillin liquid 1m L / d orally in antibiotic group. 20 days later,the pathology of pharyngeal mucosa tissue in each group was observed. Results 10 days after treatment,the general state of the animals in YYX high-dose group,low dose group and positive drug group were improved,and were better in YYX high-dose and low-dose groups. In YYX lowdose and high-dose group,the local symptoms were improved significantly,the symptoms of reduced feeding rate were disappeared,and the feeding rate was reduced and the restlessness symptoms had no improved in positive drug group. Observation of frontal mirror showed that the pharyngeal tissue was closed to the normal animal,although parted of mild chronic congestion,but mucosal showed ruddy color. After 20 days of treatment,YYX high-dose group and low-dose group and pathological manifestations of mucosal tissue was closed to the black control group,epithelial hyperplasia was not obvious,and no inflammatory cell infiltration,inherent film changed little,muscle fibers arranged more rules,these were closed to the blank control group. While the pathology change of antibiotics group was not big,and it was approximate to the model group. Conclusion YYX achieve a significant effect on chronic pharyngitis by anti-inflammatory,reducing inflammatory exudation,inhibit

关 键 词：咽炎消合剂 慢性咽炎 动物模型 咽黏膜 

分 类 号：R-332[医药卫生]