miRNA-449b在胃癌组织中的表达及与淋巴结转移的关系  

Expression of miRNA-449b in gastric cancer and lymph node metastasis

作　　者：林爱琴[1] 汪根莲 段仁杰[1] 李铁臣[1] 

机构地区：[1]皖南医学院分子生物学研究室,安徽芜湖241002

出　　处：《皖南医学院学报》2015年第2期113-116,共4页Journal of Wannan Medical College

基　　金：皖南医学院中青年科研基金项目(WK201309)

摘　　要：目的:通过对胃癌组织及癌旁组织中miRNA-449b表达的比较,分析miRNA-449b与胃癌发生及淋巴结转移的关系,为进一步探讨其在胃癌中的作用机制提供依据。方法:收集30例胃癌及癌旁组织标本,提取组织总RNA;茎环法反转录成c DNA;用实时荧光定量PCR方法检测miRNA-449b在组织中的表达水平。结果:相比于癌旁组织,miRNA-449b在胃癌组织中有23例(76.67%)表达下调,7例(23.33%)表达上调,其平均表达水平低于癌旁组织,差异有统计学意义(t=2.395,P=0.023,P<0.05)。miRNA-449b在17例有淋巴结转移的样本中16例表达下调(94.12%),13例无淋巴结转移的样本只有7例表达下调(53.85%),淋巴结转移组与无淋巴结转移组miRNA-449b的下调比例,经卡方检验,差异有统计学意义(P=0.025,P<0.05)。结论:miRNA-449b与胃癌的发生及淋巴结转移相关。Objective：To investigate the expression of miRNA-4d-9b in gastric cancer and its adjacent tissue and incidence of lymph node metastasis for elucidating the mechanisms of this gene in development of gastric cancer. Methods ： The specimen together with adjacent tissues was obtained in 30 pa- tients of gastric carcinoma. Total RNA was extracted from these tissues, and reversely transcribed into cDNA by stem-loop technique. Real-time fluorescence quota PCR was used to determine miRNA-449b expression in the tissues. Results： Expression of miRNA-449b was down-regulated in 23 cases （76.67 % ） and up-regulated in 7 （23.33 % ）. The expression level was generally lower than that of the adjacent tissues （ t = 2. 395,P = 0. 023, P 〈 0.05 ）. The miR- NA-449b expression was down-regulated in 16 of 17 cases with lymph node metastasis （94.12%）, and in 7 of 13 without metastasis （53.85 % ）, which suggested significant difference by Chi-square test （ P = 0. 025, P 〈 0.05 ）. Conclusion ： miR-449b is associated with the development of gastric cancer and presence of lymph node metastasis.

关 键 词：胃癌 miRNA-449b 淋巴结转移 

分 类 号：R735.2[医药卫生—肿瘤]