高强度聚焦超声阻断金地鼠颊囊白斑癌变的实验研究  

Experimental study on high intensity focused ultrasound in blocking leukoplakia carcinogenesis of golden hamster cheek pouches

作　　者：唐宇英[1,2] 邓军[1,3] 陈方淳[1,4] 

机构地区：[1]口腔疾病与生物医学重庆市重点实验室,重庆401147 [2]重庆医科大学附属口腔医院牙体牙髓科,400015 [3]重庆医科大学附属口腔医院儿童牙病科,401147 [4]重庆医科大学附属口腔医院口腔黏膜科,401147

出　　处：《重庆医学》2015年第13期1744-1746,共3页Chongqing medicine

基　　金：重庆市科委应用基础研究项目(CSTC;2010BB5364);2013年重庆高校创新团队建设计划资助项目

摘　　要：目的评价高强度聚焦超声(HIFU)治疗口腔白斑的有效性及可行性。方法将30只金地鼠分为两组,A组(n=5):为空白对照组,颊囊涂布生理盐水;B组(n=25)两侧颊囊涂布0.5%二甲基苯并蒽(DMBA)丙酮液,每周3次,连续5周,构建白斑动物模型。左侧为超声治疗侧,HIFU剂量参数为功率4周,时间10min,右侧为模型对照侧,不进行超声治疗,均于超声治疗后6周时处死。取颊囊标本进行大体病理观察、光学显微镜下病理切片变化比较。结果 25只金地鼠颊囊超声治疗侧(左侧)白斑、上皮异常增生和肿瘤发生率均低于模型对照侧(右侧),高于空白对照组,差异有统计学意义(P<0.05)。结论 HIFU治疗能有效降低金地鼠颊囊白斑的发生率并阻断其癌变。Objective To evaluate the effectiveness and feasibility of high intensity focused ultrasound（HIFU） in the treat- ment of oral leukoplakia. Methods Thirty golden hamsters were randomly divided into 2 groups. The group A（n= 5） as the blank control group was coated with saline at cheek poueh;the group B（n= 25） as the experimental group was coated with 0.5 % dimeth- ylbenzanthracene （DMBA） acetone solution at the cheek pouch, three rimes a week,for 5 consecutive weeks to set up the animal models of leukoplakia. The left side was treated with HIFU,with the dose parameter of 4 W and 10 min,and the right side was the model control side without ultrasonic treatment. All animals were sacrificed after 6- week ultrasonic treatment. The cheek pouch specimens were performed the gross pathological and microscopical comparison on the pathological sections. Results The occur- rence rates of leukoplakia, epithelial dysplasia and tumor in the ultrasonic treated side （left side） of cheek pouches in 25 golden ham- sters were significantly lower than those in the model control side （right side） and higher than those in the blank control group, the differences were statistically significant（P〈0.05）. Conclusion The HIFU therapy can effectively reduce the occurrence rates of leukoplakia in golden hamster cheek pouches and block its carcinogenesis.

关 键 词：高强度聚焦超声 白斑 金地鼠 

分 类 号：R781.5[医药卫生—口腔医学] R454.3[医药卫生—临床医学]