大蒜素联合阿霉素诱导胃癌SGC-7901细胞凋亡作用  

Combination of Allicin and Adriamycin Inhibits Proliferation and Induces Apoptosis in Human Gastric SGC-7901 cell

作　　者：张希锐[1] 邵淑丽[1] 李凤英[1] 张伟伟[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006

出　　处：《天然产物研究与开发》2014年第A02期309-312,共4页Natural Product Research and Development

基　　金：黑龙江省自然科学基金(C200624,C201241);黑龙江省教育厅科学技术项目(11511447,12511611);黑龙江省高校农产品加工重点实验室开放项目

摘　　要：研究大蒜素联合阿霉素诱导胃癌SGC-7901细胞凋亡的作用。台盼蓝拒染法检测细胞增殖活性，通过光镜、荧光显微镜、透射电镜、DNA琼脂糖凝胶电泳、流式细胞术控测不同浓度的大蒜素和阿霉素单独及联合作用对SGC-7901细胞增殖的影响。结果表明，大蒜素与阿霉素联合作用可增强诱导细胞凋亡。To evaluate the anti-tumor effect of Allicin combining with ADM on the human gastric cancer cells line SGC- 7901. The apoptosis of SGC-7901 cells induced by different concentrations of allicin and ADM was applied alone or together, to gastric cancer ceils SGC-7901 was analyzed by visible light, fluorescence and transmission electron microscope （TEM） ,agrose gel electrophoresis of DNA;Distribution of cell apoptosis was analyzed using flow cytometry. The reanlt show that Comparred with the control, allicin and ADM and significantly inhibited the proliferation of SC, C-7901 cells. The combination of allicin and ADM was found to be more effective in inhibiting growth.

关 键 词：大蒜素 阿霉素 SGC-7901 细胞增殖 

分 类 号：Q291[生物学—细胞生物学]