大豆水提物对四氯化碳诱导的肝损伤小鼠肝脏抗氧化活力的影响  

Antioxidant Effect of Soybean Water Extracts( SWEs) on Acute Liver Injury induced by CCl_4 in Mice

作　　者：陈玉胜[1] 陈全战[1] 

机构地区：[1]南京晓庄学院生物化工与环境工程学院,江苏南京211171

出　　处：《大豆科学》2015年第2期341-344,共4页Soybean Science

基　　金：南京晓庄学院校级重点学科基金项目(XZZDXK201203)

摘　　要：为研究大豆水提物(SWEs)对四氯化碳(CCl4)所致急性肝损伤小鼠的保护作用以及对肝脏抗氧化活力的影响。将60只试验小鼠随机分为正常对照组、模型组、SWEs低(50 mg·kg-1),中(100 mg·kg-1),高(150 mg·kg-1)剂量组及阳性对照组(联苯双脂,100 mg·kg-1)。每天灌胃给药1次,连续7 d。末次给药1 h后,除正常对照组外的其余5组采用10%CCl42.0 m L·kg-1剂量一次性腹腔注射,建立小鼠急性肝损伤模型。6 h后摘眼球取血及肝脏,计算各组小鼠的肝指数,比色法测定血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性,及总胆红素(TBIL)和白蛋白(ALB)水平,检测肝组织中丙二醛(MDA)和还原型谷胱甘肽(GSH)水平及一氧化氮合酶(NOS)活性。结果表明:模型组小鼠肝重和肝指数、血清ALT和AST活性及TBIL水平、肝组织MDA水平及NOS活性均显著高于对照组;而血清ALB及肝组织GSH水平显著低于对照组。与模型组相比,中、高剂量的SWEs及联苯双脂显著降低CCl4所致急性肝损伤小鼠的肝重和肝指数、血清ALT和AST活性及TBIL水平、肝组织NOS活性和MDA水平,并显著提高血清ALB及肝组织GSH水平。大豆水提物对CCl4所致小鼠急性肝损伤具有保护作用,其机制可能与抗氧化作用有关。To explore the protective effects of soybean water extracts （SWEs） on acute liver damage induced by carbon tetra- chloride （ CC14 ） in mice. Sixty mice were randomly divided into control group, model group and SWEs groups at dose of 50, 100 and 150 mg＇kg-~ , and positive control group （bifendate, 100 mg.kg-~ ）. All the mice were intragastrically administrated the corresponding agents respectively once a day for 7 days. One hour after the administration, 10% CC14 2. 0 mL＂ kg-~ was given to mice in ＂all groups except the control group, with a single intraperitoneal injection to establish the acute liver injury mouse model. Six hours later, mice were sacrificed, and the liver index, alanine aminotransferase （ALT） and aspartate amin- otransferase （AST） activities, total bilirubin （TBIL） and albumin （ALB） level in serum were detected; the malondialdehyde （MDA） and glutathione （GSH） levels, and nitric oxide synthase （NOS） activity in liver tissue were also analyzed. Results indicated that CC14 significantly increased the liver weight, liver index, and levels of ALT, AST and TBIL in serum, MDA and NOS in liver tissue; while levels of ALB in senlm and GSH in liver tissue were remarkably reduced in model group com- pared with normal group. Compared with model group, 100 and 150 mg.kg-1 SWEs and bifendate notably decreased liver weight and index, levels of ALT, AST and TBIL in serum, NOS activity and MDA level in liver tissue in acute liver injury mice induced by CC14 treatment; Meanwhile significantly increased levels of ALB in serum and GSH in liver tissue. As a con- clusion, SWEs has a significant protective effect on CCI-induced liver injury＂ mice, which might be related to the anti-oxidant function.

关 键 词：大豆水提物 肝损伤 抗氧化 联苯双脂 

分 类 号：R285.5[医药卫生—中药学]