药对广藿香-陈皮的挥发油提取工艺及其化学成分的GC-MS研究  

Optimization of volatile oil extraction from herbal pair Pogostemon cablin-Citrus reticulata and GC-MS analysis

作　　者：陈琳[1] 梁咏倩 宋粉云[1] 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《广东药学院学报》2015年第2期190-197,共8页Academic Journal of Guangdong College of Pharmacy

基　　金：上海市药物(中药)代谢产物研究重点实验室开放课题(2012SHDX1002)

摘　　要：目的研究药对广藿香-陈皮的挥发油提取工艺,并分析广藿香、陈皮配伍前后的挥发油成分。方法基于单因素试验基础,采用响应面分析法优化水蒸气蒸馏法提取挥发油的工艺条件。并利用气相色谱-质谱(GC-MS)联用法和自动质谱退卷积定性系统(AMDIS),结合程序升温保留指数(PTRI)对药对及各单味药材的挥发油成分进行鉴定及质量分数比较。结果提取广藿香和陈皮挥发油最佳工艺条件为:粉碎后药材过60目筛,10倍液料比,提取5 h。通过GC-MS分析确定药对广藿香-陈皮、单味药广藿香、陈皮各挥发油中的134、121和85个成分,分别占总质量分数的84.01%、82.80%和78.79%。结论提取广藿香和陈皮挥发油的最佳工艺合理可行;广藿香、陈皮药对配伍对所提取挥发油的成分和质量分数都有较大的影响。Objective To investigate the extraction condition of volatile oil distilled from herbal pair （HP） of Pogostemon cablin （Blanco） Benth. （PCB） and Citrus reticulata Blanco. （CRB） and discuss the influence of drug compatibility on the components of the volatile oil in PCB, CRB and the HP PCB-CRB.Methods The steam distillation extraction of volatile oil from the herbal pair was optimized for maximum oil yield by response surface methodology based on single factor experiments, analyzed by gas chromatography-mass spectrometry with automated mass spectral deconvolution and identification system （GC-MS-AMDIS） based on the temperature-programmed retention index （PTRI）. The chemical fingerprints were compared to identify the main chemical constituents, and the relative concentration of each component was determined by peak area normalization.Results The optimal conditions of extraction process were as follows： sifted with 60 mesh sieve,solvent ratio of 10：1 and extracting for 5 h. 134,121 and 85 chemical components were identified.The identified components were 84.01%,82.80% and 78.79% of the extractions from HP,PCB and CRB, respectively.Conclusion The optimal extraction conditions of volatile oil distilled from PCB and CRB are steady and feasible, and the compatibility of the herbal pair has a great influence on components and contents in the volatile oil of PCB and CRB.

关 键 词：药对 挥发油 响应面法 水蒸气蒸馏法 气相色谱-质谱 保留指数(PTRI) 

分 类 号：R284.1[医药卫生—中药学]