ApoE^(-/-)小鼠动脉粥样硬化模型的建立  被引量:22

The Establishment of Atherosclerosis Model in ApoE^(-/-) Mice

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作  者:欧海龙[1] 张礼林[1] 何晓兰[1] 李红梅[1] 雷霆雯[1] 

机构地区:[1]贵阳医学院生物化学与分子生物学教研室,中国贵州贵阳550004

出  处:《生命科学研究》2015年第2期141-144,共4页Life Science Research

基  金:贵阳医学院博士启动基金项目(2012006)

摘  要:ApoE-基因敲除小鼠(ApoE-/-)经含有21%脂肪和0.15%胆固醇的高脂饲料喂食12周后进行各项血脂胆固醇水平检测,以及整体主动脉油红O染色与主动脉根部病理切片油红O染色等动脉粥样硬化病理分析。结果显示经过高脂诱导的ApoE-/-小鼠的血浆总胆固醇和甘油三酯水平均比未经饮食诱导的ApoE-/-小鼠、经同样饮食处理的野生型小鼠以及未经处理的野生型小鼠均显著升高(P<0.05);低密度脂蛋白-胆固醇水平与野生型(正常饮食组和高脂组)相比升高了近3倍多;高脂诱导ApoE-/-小鼠的主动脉斑块面积占整体主动脉面积的65%,显著高于ApoE-/-小鼠的正常饮食组(21%)(P<0.05),同时主动脉根部的血管壁明显增厚,管腔变窄。实验结果表明通过高脂饲料饮食诱导,成功建立了动脉粥样硬化模型小鼠,可为下游的药物筛选、基因治疗以及动脉粥样硬化机理的体内研究提供理想的实验材料。The ApoE gene knockout mice (ApoE^-/-) were fed with a high-fat (HF) diet containing 21% fat and 0.15% cholesterol for 12 weeks to establish atheroselerosis model. After diet induction, the ApoE^-/- mice were sacrificed and plasma total cholesterol (TC), triglyeeride (TG), low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein-cholesterol (HDL-C) levels were measured. The whole aortas and se- quential sections of the aortic root were stained with oil red O. Results showed that the plasma levels of TC and TG from HF diet induced ApoE^-/- mice were both dramatically higher than normal diet(ND)-fed ApoE^-/- mice as well as wild-type mice fed with normal diet or HF diet (P 〈0.05). The contents of LDL-cholesterol in plasma were elevated by three-fold compared with wild-type (ND and HFD). Atherosclerotic lesion sizes were significantly increased in whole aorta (65%) as compared with normal diet ApoEu- mice (21%). Similar result was obtained from cross-sections of the aortic root analysis. The results demonstrated that HF diet treatment greatly enhanced atherosclerosis development in ApoE^-/- mice. The establishment of atherosclerosis model mice provides a valuable tool for drug screen, gene therapy and even in vivo mechanism analysis in atherosclerosis disease.

关 键 词:APOE^-/-小鼠 动脉粥样硬化 胆固醇 血脂 主动脉 

分 类 号:R394[医药卫生—医学遗传学]

 

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