TLRs／NF—κB信号通路在大鼠溃疡性结肠炎表达的相关性分析  

Analysis of the Expression of TLRs/NF-κB Signaling Pathway in Rat Ulcerative Colitis

作　　者：迟玉花[1] 赵刚[1] 乔翠霞[1] 刘鹏林[1] 高海瑞[1] 

机构地区：[1]青岛大学附属医院,山东青岛266555

出　　处：《中国肛肠病杂志》2015年第3期7-9,共3页Chinese Journal of Coloproctology

摘　　要：为观察Toll样受体／核转录因子-κB（TLRs／NF-κB）信号通路在溃疡性结肠炎大鼠结肠中表达的相关性，选取成年健康Wistar大鼠40只，分成实验组30只与对照组10只。对照组正常饲养，实验组根据右旋葡聚糖硫酸钠（DSS）加激怒法建立溃疡性结肠炎模型。造模成功后，采用免疫组化方法观察对比两组结肠组织中TLR-2、TLR-4、NF-κB表达的差异及观察TLR-2、TLR-4和NF_KB的相关性。结果显示，实验组中TLR-2、TLR-4、NF-κB的表达均较对照组中高，且TLR-2、TLR-4、NF-κB的表达与病理分级呈相关性。结果表明，TLRs／NF-κB信号通路异常导致肠黏膜的炎症反应，并与病情严重程度相关。This study was to observe the expression of Toll-like receptors/nuclear transcripation factor-κB （TLRs/NF-κB） signaling pathway in rats with ulcerative colitis,40 healthy adult Wistar rats were selected and divided into experimental group （ n = 30）and control group（ n = 10）.Control group received normal feeding, experimental group were established model by dextran sulphate sodium（DSS） plus irritating meth- ods.Immunohistochemistry was used to observe the expression of TLR-2, TLR-4 and NF-κB difference, and their correlation in rectal tissue.The results showed that the expression of TLR-2,TLR-4 and NF-κB was relatively high in experimental group than in control group;and there was a correlation between the expression of TLR-2,TLR-4,NF-κB and pathological grade.It is concluded that TLRs/NF-κB signaling pathway abnormal can induce inflammation in intestinal mucosa,which is associated with disease severity.

关 键 词：溃疡性结肠炎 大鼠 Toll样受体/核转录因子-κB 表达 

分 类 号：R574.62[医药卫生—消化系统]