响应面法优化超声辅助提取南板蓝根多糖工艺  

Optimization of Ultrasound-assisted Extraction Technology of Baphicacanthis cusiae Roots Polysaccharide by Response Surface Methodology

作　　者：鲁轮 郑少燕[2] 赵晓勤[1] 万绵洁 张靖年 张丹雁[1] 

机构地区：[1]广州中医药大学,广东广州510006 [2]南方医科大学附属佛山妇幼保健院,广东佛山528000

出　　处：《广州中医药大学学报》2015年第3期508-512,578,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金："十二.五"国家科技支撑计划项目(编号:2010B090400529);广东省科技计划资助项目(编号:2010B030700067)

摘　　要：【目的】采用优选超声波法提取南板蓝根多糖的最佳工艺条件,为南板蓝根多糖的开发研究提供理论依据。【方法】选定提取温度、时间及水料比作为影响因素,以南板蓝根多糖提取率为评价指标。在单因素试验的基础上,通过3因素3水平Box-Behnken设计试验,建立多糖提取率的二次多项式回归方程,采用响应面回归分析得到优化组合条件。【结果】最佳提取工艺为提取温度60℃,提取时间35 min,水料比24.5∶1(V∶m,m L/g),南板蓝根多糖的提取率为83.7 mg/g。【结论】此方法具有简单、快捷、高效等优点,可为南板蓝根多糖的综合开发应用奠定理论基础。Objective To optimize the processing condition of the extraction of Baphicacanthis cusiae （ Nees） Bremek. roots polysaccharides （ BCP） by ultrasound technology, and to provide the basis for the further development and study of BCP. Methods The extraction temperature, extraction time, liquid-solid ratio were used as the influence factors, and the yield of polysaccharides from Baphicacanthis cusiae roots was used as the evaluation index. On the basis of single-factor test, 3-factor and 3-level Box-Behnken test was designed for quadratic polynomial regression equation of the yield of polysaccharides. And then the response surface methodology was used for the optimization of process condition. Results The optimum extraction condition was as follows： extraction temperature was 60℃, extraction time was 35 min, and liquid-solid ratio was 24.5∶1 （V∶m, mL·g-1）. Under the optimal condition, the yield of polysaccharides was 83.7 mg·g-1. Conclusion The method is simple, rapid and highly efficient, which will provide experimental basis for the development and application of Baphicacanthis cusiae roots polysaccharides.

关 键 词：南板蓝根/分离和提取 多糖/分析 响应面分析法 超声辅助提取 分光光度法  紫外 

分 类 号：R284.2[医药卫生—中药学]