CXCR4 shRNA对U87胶质瘤细胞周期和凋亡的影响  

Effects of CXCR4 sh RNA on cell cycle and apoptosis of U87 glioma

作　　者：袁宏[1] 冯虎[2] 家秀秀 姚晓晴 王溪[2] 

机构地区：[1]四平市第一人民医院神经外科,吉林四平136001 [2]吉林大学第二医院放射科,吉林长春130041 [3]吉林大学公共卫生学院,吉林长春130041

出　　处：《中国热带医学》2015年第1期25-27,共3页China Tropical Medicine

摘　　要：目的探讨下调CXCR4的表达对U87胶质瘤细胞周期和凋亡的影响,为CXCR4 sh RNA治疗胶质瘤提供实验依据。方法设计合成CXCR4的特异性sh RNA,采用脂质体转染U87细胞株。实验将细胞分为空白对照组(A组),阴性对照组(转染非特异载体p GPU6/GFP/NC组)(B组),实验组(转染CXCR4-sh RNA组)(C组)共三组,用RT-PCR检测各组细胞CXCR4基因表达情况,用流式细胞术检测细胞周期分布和细胞的凋亡情况。结果成功构建了CXCR4的特异性sh RNA。C组的U87细胞CXCR4 m RNA为(42.5±4.6)%,显著低于B组的(65.4±5.9)%和A组的(69.6±7.4)%(P<0.05),G0/G1期细胞比例增加(39.4±1.7)%与(25.8±1.3)%和(20.3±1.2)%,G2/M期细胞比例相应下降(20.2±1.6)%与(30.1±1.2)%和(26.4±1.3)%;S期细胞比例相应下降(46.5±2.7)%与(55.7±2.8)%和(54.9±2.6)%,凋亡细胞率为(18.68±0.44)%明显高于A组(5.14±0.23)%和B组(4.87±0.16)%(P<0.05)。结论 CXCR4 sh RNA能够有效下调U87细胞CXCR4基因表达,诱导细胞凋亡和细胞周期阻滞,从而抑制细胞的增殖,提示CXCR4在胶质瘤发展过程中具有重要作用。Objective To investigate the effects of expression of CXCR4 sh RNA on glioma cell cycle and apoptosis of U87 glioma and to provide experimental basis for treatment of glioma with CXCR4 sh RNA. Methods U87 cells was transfected byspecific CXCR4 sh RNA through lipofectamine. The glioma cells were divided into 3 groups： Group A（blank control）, Group B（transfected non-specific p GPU6/GFP/NC group） and group C（ CXCR4 sh RNA）.CXCR4 gene expression of cells was analyzedby RT- PCR. Glioma cell cycle and apoptosis were detected by flow cytometry. Results Compared with transfected non-specific p GPU6/GFP/NC group and untransfected group, CXCR4 m RNA of U87 cells transfected CXCR4- sh RNA wassignificantly lower（42.5±4.6） %vs（65.4±5.9）% and（ 69.6±7.4）%（P〈0.05）, the proportion of G0/G1 phase of cells increased（39.4±1.7）%vs（25.8±1.3）% and（ 20.3±1.2）%, the proportion of G2 / M and S phase of cells corresponding declined（20.2±1.6）%vs（30.1±1.2）% and（26.4±1.3）%;（46.5±2.7）%vs（55.7±2.8）% and（54.9±2.6）%,the rate of apoptotic cells was significantlyincreased（18.68±0.44）% vs（5.14±0.23）% and（4.87±0.16）% than that of the blank control（P〈0.05）. Conclusions CXCR4 sh RNA could effectively reduced CXCR4 gene expression of U87 cell, induced apoptosis and cell cycle arrest, therebyinhibited cell proliferation. Those results suggested that CXCR4 plays an important role in the development of gliomas.

关 键 词：CXCR4基因 U87细胞 细胞凋亡 细胞周期 

分 类 号：R739.41[医药卫生—肿瘤]