基于诱导多潜能干细胞Alport综合征的蛋白质组学研究  

Proteomic analysis of alport syndrome based on induced pluripotent stem cells

作　　者：喻祥琪 黄建溶 林小聪[3] 陈文标[1] 戴勇[1] 

机构地区：[1]暨南大学第二临床学院,深圳市人民医院临床医学研究中心,广东深圳518020 [2]深圳市第三人民医院肾脏内科,广东深圳518112 [3]广东医学院生物化学研究所,广东湛江524023

出　　处：《中国热带医学》2015年第2期133-136,144,共5页China Tropical Medicine

基　　金：深圳市知识创新计划(No.JCYJ20140416122812045)

摘　　要：目的筛选并鉴定Alport综合征(AS)患者与健康对照者(NC)差异性表达蛋白质,寻找AS的生物标记物。方法收集AS组与NC组的尿液,从尿液中分离尿肾脏管细胞,将尿肾脏管细胞诱导分化成多潜能干细胞(i PSCs)。运用i TRAQ技术找出两组之间差异性表达蛋白质。采用Western blot检测差异蛋白质。结果共鉴定出12 600条特有肽段序列,对应3 470种非冗余蛋白质。在两组样本中发现383种蛋白质具有差异性表达,其中差异2倍以上的蛋白质有35种,包括18种上调蛋白质和17种下调蛋白质。Western blot检测KRT14、TUBA1A、PAPSS1、UTF1、SF3B14、MTHFD2等6种差异蛋白质在各组中的表达结果与i TRAQ检测结果一致。结论筛选得到与AS发病相关的差异蛋白质,可以作为AS早期诊断和治疗的生物标记物。Objective To identify the different expression proteins in patients with Alport syndrome（AS） and healthcontrol（NC）so as to identify the biomarker of AS. Methods Aseptic middle stream of the micturition was collected in themorning from each participant. Then, the renal tubular cells were separated out from urine. The renal tubular cells werereprogrammed to generate human induced pluripotent stem cells. i TRAQ technology were used to find out the proteins whichwere significantly differentially expressed in AS and NC. And the result was confirmed by Western blot. Result Total 12 600 unique peptides were found out, of which was corresponding to a set of 3 470 proteins. Among the proteins, 383 proteins weredifferentially expressed between AS and NC, including 18 up-regulated and 17 down-regulated proteins with difference morethan twice. Six proteins（KRT14, TUBA1 A, PAPSS1,UTF1, SF3B14, MTHFD2） were confirmed by Western blot. The result wasconsistent with that of i TRAQ test. Conclusion The proteins related to a close relationship to the pathogenesis of AS couldserved as biomarker for early diagnosis and treatment.

关 键 词：ALPORT综合征 多潜能干细胞 蛋白质组学 同位素标记相对和绝对定量 

分 类 号：Q343[生物学—遗传学]