miRNA在稳定白斑和癌变白斑组织中表达的比较  

Different miRNAs expressed between progressing leukoplakias and non-progressing leukoplakias

作　　者：李月秀[1] 肖长杰[1] 杨娅[2] 

机构地区：[1]泰安市中心医院口腔科,山东271000 [2]上海交通大学附属第九人民医院

出　　处：《社区医学杂志》2015年第8期5-7,共3页Journal Of Community Medicine

摘　　要：目的通过分析癌变白斑和稳定白斑病损组织中miRNA表达谱的异常,以期发现可准确评估此类病损癌变风险的生物学标志物。方法使用Taq Man低密度miRNA芯片对稳定白斑和癌变白斑进行分子生物学检测分析。结果同稳定白斑相比较,癌变白斑中miRNAs的表达发生了显著改变,差异有统计学意义(P<0.05),其中13个miRNAs的表达下调(miR-99a-5p、miR-99b-5p、miR-144、miR-100-5p、miR-125-5p、miR-181b、miR-181a、miR-521-3p、miR-339-5p、miR-15a-5p、miR-150-5p、let-7a-5p、miR-9),12个miRNAs的表达上调(miR-10b-5p、miR-708、miR-30e-3p、miR-30a-3p、miR-21、miR-335-5p、miR-144、miR-25-3p、miR-19a-3p、miR-660-5p、miR-140-5p、miR-590-5p)。结论 miRNA生物标志物的检测对稳定白斑和癌变白斑病变的诊断具有重要的意义。Objective To assess the potential of a progress risk of oral precancerous lesions through the analysis of abnor- mal expression of miRNA. Methods Used miRNA microarray to profile progressing low grade dysplasia and oral premali- gant lesions,which was in order to do molecular biology detection analysis. Resulls Compared with low grade dysplasia, o- ral premaligant lesions had a significant change with express of miRNA（ P 〈 0.05 ）. Leukoplakias,with up- regulations of miR -99a -Sp,miR -99b - 5p,miR - 144,miR - 100 - 5p,miR - 125 - 5p,miR - 181b,miR - 181a,miR -521 -3p, miR - 339 - 5p ,miR - 15a - 5p, miR - 150 - 5p,let - 7a - 5p, miR - 9, and down - regulations of miR - 10b - 5p, miR - 708,miR - 30e - 3p,miR - 30a - 3p,miR - 21, miR - 335 - 5p, miR - 144, miR - 25 - 3p, miR - 19a - 3p,miR - 660 - 5p,miR - 140 - 5p,miR -590 -Sp. Conclusions It is of great significance with diagnose of low grade dysplasia and oral premaligant lesions by miRNA biomarker detection.

关 键 词：口腔白斑 癌前病变 MIRNA 

分 类 号：R739.85[医药卫生—肿瘤]