慢性心理应激对人卵巢癌移植瘤中Prdx1和Prdx3蛋白的影响  

Effects of chronic psychological stress on Prdx1 and Prdx3 proteins on human ovarian cancer xenograft

作　　者：高军[1] 朱虹[1] 高国兰[2] 

机构地区：[1]江西省人民医院妇科,南昌330006 [2]航空总医院妇科,北京100012

出　　处：《广东医学》2015年第7期989-992,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30860301);江西省科技计划项目(编号:20123BBG70231)

摘　　要：目的探讨慢性心理应激对肿瘤发生发展的影响及其可能机制。方法将裸鼠随机分成应激组及对照组,每组10只;两组均皮下注射SKOV3细胞建立荷人卵巢癌皮下移植瘤模型。应激组裸鼠采用束缚应激法进行应激,构建荷人卵巢癌裸鼠慢性心理应激模型;而对照组整个实验中不接受应激。观察两组移植瘤的生长状况;采用蛋白组学方法筛选、鉴定、验证应激组与对照组移植瘤之间的差异蛋白;分析典型差异表达蛋白与肿瘤生长的可能联系。结果应激组裸鼠的移植瘤生长速度比对照组快,最终瘤重(1.717±0.571)g明显高于对照组的(1.083±0.286)g(P<0.05);两组移植瘤中存在数百种差异表达蛋白,有20个蛋白质斑点变化超过2倍,其中Prdx1和Prdx3蛋白在应激组中表达上调极其明显(P<0.05)。结论慢性心理应激可能是通过Prdx1激活NF-κB通路促进卵巢癌的进展,Prdx1和Prdx3可能成为治疗卵巢癌的靶点。Objective To investigate the role of psychological stress in carcinogenesis and progression of ovarian cancer.Methods Nude mice were randomly divided into stress and control groups （n=10）.Ovarian carcinoma xeno-graft model was established using SKOV3 subcutaneously injection in both stress and control groups.Stress mouse models were constructed using the restraint stress method in stress group.The growth of xenograft was observed.The differentially expressed proteins between stress group and control group were located, identified, and verified by proteomics methods. Combined with preliminary experiments, the possible associations between typical different expression proteins and tumor growth were analyzed.Results The growth of xenograft in stress group was faster than that in control group, with signifi-cantly heavier tumor weight （1.717 ±0.571 g vs 1.083 ±0.286 g, P〈0.05）.There were hundreds of differentially ex-pressed proteins in xenograft between stress group and control group, as 20 protein spots were changed by over two times, among which Prdx1 and Prdx3 proteins were significantly up-regulated in stress group （P〈0.05）.Conclusion Chronic psychological stress could promote the progression of ovarian carcinoma activating NF-κB pathway via Prdx1, suggesting Prdx1 and Prdx3 as potential targets for the treatment of ovarian cancer.

关 键 词：卵巢肿瘤 心理应激 过氧化物酶1 过氧化物酶3 

分 类 号：R735.35[医药卫生—肿瘤]