miR-27a对两种结肠癌细胞增殖和侵袭能力的影响  

Effect of miR-27a on the proliferation and invasion of two colorectal cancer cells

作　　者：张子雯[1] 傅小一[1] 

机构地区：[1]江西省宜春学院医学院,江西宜春336000

出　　处：《中国生化药物杂志》2015年第2期24-27,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的探讨microRNA-27a(miR-27a)对2种结肠癌细胞增殖和侵袭能力的影响。方法应用miR-27a反义寡核苷酸(antisense oligonucleotides,ASO)在体外转染结肠癌细胞系SW620和HCT116,设置转染无义序列组作为对照组。RT-PCR检测细胞miR-27a表达;MTT法检测细胞增殖;Transwell小室侵袭实验检测细胞侵袭能力;Western blot检测转染细胞FOXO1蛋白表达。结果与对照组相比,转染miR-27a ASO后结肠癌细胞系SW620和HCT116的miR-27a表达水平均明显降低(P<0.001),增殖能力和侵袭能力均受到明显抑制(P<0.01),FOXO1蛋白表达均明显增高(P<0.05)。结论抑制miR-27a表达可显著降低结肠癌细胞系SW620和HCT116的增殖能力和侵袭能力,而调控下游FOXO1蛋白表达可能是miR-27a参与结肠癌细胞增殖和侵袭的主要机制。Objective To evaluate effect of miR-27a on proliferation and invasion of colorectal cancer cells. Methods The colorectal cancer cells of SW620 and HCT116 were transfected with miR-27a antisense oligonucleotides （ASO） in vitro, and the above cells transfected with nonsense sequence were as control group. RT-PCR was used to examine the expression levels of miR-27a. MTY method was used to examine the cell proliferation. Transwell matrigel invasion test was used to examine the cell invasion. Western blot was used to examine the protein expression levels of FOXO1. Results Compared with control group, after the colorectal cancer cells of SW620 and HCT116 were transfected with miR-27a ASO, the expression levels of miR-27a significant deereased（P 〈0. 001 ）, the ability of proliferation and invasion significant inhibited（ P 〈 0. 01 ）, the protein expression levels of FOXO1 significant increased （ P 〈 0. 05 ）. Conclusion Inhibiting miR-27a expression could significantly reduce the proliferation ability and invasion ability of SW620 and HCT116. The regulation of downstream FOXO1 protein expression may be the main mechanisms of miR-27a involved in cdoreetal cancer cell proliferation and invasion.

关 键 词：miR-27a 结肠癌 增殖 侵袭 FOXO1 

分 类 号：R735.3[医药卫生—肿瘤]