HPLC法测定生姜半夏汤单煎与合煎中6-姜酚和6-姜醇含量  

Determination of 6-gingerol and 6-shogaol in ginger pinellia decoction of different decoction by HPLC

作　　者：刘芸[1] 王珂佳[2] 黄锴[3] 莫正昌[1] 

机构地区：[1]铜仁职业技术学院药学院,贵州铜仁554300 [2]贵州轻工职业技术学院化工系,贵州贵阳550025 [3]贵州理工学院干部科,贵州贵阳550003

出　　处：《中国生化药物杂志》2015年第2期168-170,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的 HPLC法测定生姜半夏汤单煎液与合煎液中6-姜酚、6-姜醇的含量变化。方法采用Amethyst C18-P柱(4.6 mm×250 mm,5μm),以甲醇-水(60∶40)为流动相,检测波长281 nm,流速0.9 m L/min,柱温25℃。结果 6-姜酚、6-姜醇在合煎液中含量均明显高于单煎液。结论生姜半夏汤使用时应采用合煎的方式制备,否则会降低6-姜酚、6-姜醇浸出的含量,影响生姜半夏汤的药效。Objective To determine the content of 6-gingerol and 6-shogaol in separately decocted and mingly decocted ginger pinellia decoction by HPLC. Methods The samples were separated on Amethyst C18-P（ 4. 6 mm × 250 mm,5 μm） column. The mobile phase was methanol-water（ 60 ∶40） at the flow rate of 0. 9 m L / min. The detection wavelength was 281 nm. The column temperature was 25 ℃. Results The content of 6-gingerol and 6-shogaol in separately decocted obviously less than that in mingly decocted ginger pinellia decoction. Conclusion Ginger pinellia decoction should be used mingly decocted. Otherwise,it will reduce the contents of 6-gingerol and 6-shogaol,affect the efficacy of ginger pinellia decoction.

关 键 词：生姜半夏汤 6-姜酚 单煎 合煎 6-姜醇 含量测定 

分 类 号：R286.02[医药卫生—中药学]