可控模拟口腔环境的改良MD-300恒化器的建立及应用  

Establish and Application a Controllable Modified MD-300 Chemostat of Anologous Oral Environment

作　　者：李德懿[1] 李宗林[2] 张建中[1] 桂晓琳 朱霖[1] 张濒[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔内科,上海200011 [2]复旦大学生命科学院遗传工程

出　　处：《临床口腔医学杂志》2002年第4期243-245,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金 (30 0 70 81 3)

摘　　要：目的　改良现有的MD -30 0恒化器 ,以适合口腔细菌复杂的生态研究。方法　在恒化器培养罐内安装一平台 ,上面放置的羟磷灰石或材料等可固定、移动或复位 ,调节恒化器的重要参数并作初步应用。结果　改良M -30 0恒化器模拟人类口腔环境的条件为 :培养基 2 .5g/L猪胃粘蛋白的TSB ,工作容量 12 0 0ml,稀释率 5 0ml/h ,搅拌速度 5 0次 /分 ,温度 37± 0 .1℃ ,pH7.0± 0 .1,气体 80 %N2 、10 %CO2 、10 %H2 。在羟磷灰石圆片表面能方便地形成人工菌斑生物膜 ,能比较不同义齿基托材料表面的细菌粘附。结论　模拟口腔环境的改良MD -30Objective The aim of this study was to modify an existent MD-300 chemostat suitable for complicated oral bacteria ecologic study. Methods The brooder of MD-300 Chemostat was modified, in which incorporated removable and replaceable hydroxyapatice (HA) disks, and the chemostat's key parameters were regulated for primaly application. Results Chemostat conditions of anologous oral enviroment were that the medium was TSB broth with 2.5 g/L porcine gastric mucin, the volume was 1200 ml, the medium flow rate adjusted to 50 ml/h, culture pH was maintained at 7.0± 0.1, temperature was 37±0.1 ℃ and air was N 2, CO 2, H 2 (80∶10∶10). Artificial plaque biofilms could conventional develop on HA dishes and bacteria adhesion to the surface of different resin denture material could be detected in the modified MD-300 chemostat. Conclusion Artificial dental plaque biofilm and oral bacteria ecologic relationship could be studied by the modified MD-300 chemostat of anologous oral environment.

关 键 词：可控模拟口腔环境 MD-300恒化器 菌斑生物膜 生态关系 口腔菌群 

分 类 号：R780.2[医药卫生—口腔医学] TH787[医药卫生—临床医学]