粉笔PM_(2.5)/PM_(10)诱导大鼠巨噬细胞产生活性氧和活性氮机理  

Mechanisms of Chalk-induced Reactive Oxygen Species and Reactive Nitrogen Species in AMs

作　　者：张月霞[1] 冯燕[1] 杨振华[1] 张全喜[1] 李瑞金[1] 董文娟[1] 耿红[1] 董川[1] 

机构地区：[1]山西大学环境科学研究所,山西太原030006

出　　处：《山西大学学报（自然科学版）》2015年第2期361-366,共6页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(21107064;21177078;11274340;21175086;2140509)

摘　　要：采用鲁米诺增强的化学发光法研究粉笔PM2.5/PM10诱导大鼠肺泡巨噬细胞(AMs)产生活性氧(ROS)和活性氮(RNS)的能力,并运用抗霉素A,超氧化物歧化酶(SOD),二苯基氯化碘盐(DPI)和左旋-N-硝基精氨酸甲酯(L-NAME)等抑制剂来确定ROS和RNS产生来源。结果发现粉笔PM2.5/PM10能诱导AMs产生化学发光,1mmol/L L-NAME可显著抑制粉笔PM2.5/PM10诱导AMs的化学发光。CaSO4/CaCO3PM2.5或PM10也可浓度依赖地诱导AMs产生化学发光,且CaSO4/CaCO3相同组分的颗粒粒径越小,诱导化学发光能力越强。CaCO3PM2.5或PM10诱导化学发光的能力远远强于同粒径CaSO4PM2.5或PM10,结果具有统计学意义。研究也发现,抗霉素A,SOD,DPI和L-NAME可显著抑制CaSO4/CaCO3PM2.5或PM10诱导AMs化学发光。这些结果提示粉笔诱导AMs生成的ROS可能源于细胞内NADPH氧化酶和线粒体complexⅢ,RNS则源于细胞内一氧化氮合酶激活。CaSO4和CaCO3是粉笔PM2.5/PM10诱导产生ROS/RNS的主要因素。The abilities to produce ROS/RNS in rat AMs exposed to chalk particles were investigated by using a luminol-enhanced chemiluminescence（CL）method and the effects of various inhibiters on CL in AMs exposed to particles were also examined.We found that chalk particles induced CL in AMs,which was significantly inhibited by 1mmol/L L-NAME.CaSO4/CaCO3PM2.5or PM10 induced CL in a dose-dependent manner.With regard to CaSO4 or CaCO3,the smaller the particle size,the stronger chemiluminescence.Also,CaCO3 particles markedly induced the stronger CL in AMs than CaSO4.Moreover,CL in AMs exposed to CaSO4 or CaCO3 were inhibited significantly by antimycin A,SOD,DPI and L-NAME.The results suggested that chalk-induced ROS in AMs may derive from NADPH oxidase in cytoplasmic membrane and complex Ⅲ in mitochondria and RNS may result from NOS activation in AMs.Furthermore,chalk-induced ROS/RNS in AMs is closely related to the presence of CaSO4 and CaCO3in chalk.

关 键 词：粉笔PM2.5/PM10 化学发光 活性氧 活性氮 

分 类 号：X174[环境科学与工程—环境科学] R994.6[医药卫生—毒理学]