Expression of estrogen receptor (ER) -α and -βtranscripts in the neonatal and adult rat cerebral cortex, cerebellum, and olfactory bulb  

作　　者：GuoXZ SuJD 

机构地区：[1]DepartmentofBiochemistryandMolecularBiology,SecondMilitaryMedicalUniversity,Shanghai200433 [2]SchoolofLifeSciencesandMedicalEngineering,TongiiUniversity,Shanghai200092,China

出　　处：《Cell Research》2001年第4期321-324,共4页细胞研究（英文版）

摘　　要：In the present study expression of estrogen receptor subtype -alpha (ERalpha) and -beta (ERbeta) in the cerebral cortex, cerebellum, and olfactory bulb was investigated and compared between neonatal (1 to approximately 3-days-old) and adult (250 to approximately 350 g) rats, using reverse transcription-polymerase chain reaction (RT-PCR). No ERalpha transcripts were detectable in the adult cerebellum and olfactory bulb, whereas very weak expression of ERalpha was present in the adult cerebral cortex. No significant difference in ERbeta transcripts was detectable between the neonatal and adult rats. While transcripts for both ER subtypes were co-expressed in these brain areas of neonatal rats, although ERalpha expression was significantly weaker than ERbeta. Even in the cerebral cortex known to contain both ER subtypes in adult rats, ERalpha transcripts in neonatal rats were much higher than in adult. These observations provide evidence for the existence of different expression patterns of ERalpha/ERbeta transcripts in these three brain areas between the neonatal and adult rats, suggesting that each ER subtype may play a distinct role in the regulation of differentiation, development, and functions of the brain by estrogen.

关 键 词：ANIMALS Animals  Newborn Brain CEREBELLUM Cerebral Cortex Estrogen Receptor alpha Estrogen Receptor beta Female Male Olfactory Bulb RNA  Messenger RATS Rats  Sprague-Dawley Receptors  Estrogen Research Support  Non-U.S. Gov't Transcription  Genetic 

分 类 号：Q579.13[生物学—生物化学] R338[医药卫生—人体生理学]