补骨脂中补骨脂素和异补骨脂素测定  

Improvement in determination of psoralen and sopsoralen from Psoralea corylifolia L.

作　　者：赵婷[1] 朱汀滢 吴斌[1] 

机构地区：[1]扬子江药业集团上海海尼药业有限公司,上海201318

出　　处：《中成药》2015年第5期1036-1040,共5页Chinese Traditional Patent Medicine

摘　　要：目的根据《欧洲药典》质量标准要求,改进补骨脂中补骨脂素和异补骨脂素的HPLC测定方法。方法补骨脂以甲醇提取,YMC-Triart C18色谱柱（4 mm×250 mm,5μm）,流动相为乙腈-水（35∶65）,等度洗脱,体积流量为1 m L/min,检测波长为246 nm。结果补骨脂素和异补骨脂素在10~100μg/m L范围内成线性,并在Merck-Li Chrospher RP-18（4 mm×250 mm,5μm）和Waters Spherisorb ODS2 column（4 mm×250 mm,5μm）色谱柱上可重复实验结果,两成分的定量测定结果偏差分别为1.50%和1.33%。7个省和缅甸产共16批补骨脂样品中的补骨脂素和异补骨脂素含有量在1.11%（云南）和0.29%（广西）之间。结论建立的方法可符合欧盟植物药申报专属性、精密度（日间、日内）、准确度的要求。AIM To establish an HPLC method for determining the contents of psoralen and isopsoralen from Psoralea corylifolia L. in agreement with European Pharmacopoeia. METHODS The analysis of P. corylifolia L methanolic extract was carried out on YMC-Triart C18（4 mm × 250 mm,5 μm） column with acetonitrile- water（35 ∶ 65） as mobile phase in isocratic elution manner and 1 m L/min of flow speed,and detection wavelength was set at 254 nm. RESULTS The linear relationships of psoralen and isopsoralen were in the range from 10 to 100μg /m L. The same process was repeated on Merck- Li Chrospher RP-18（4 mm × 250 mm,5 μm） and Waters Spherisorb ODS2 column（4 mm × 250 mm,5 μm）,their bias were restricted to the limit of 1. 50% and 1. 33%,respectively. The contents of psoralen and isopsoralen collected from seven provinces in China and Burma fell within the range of 1. 11%（Yunnan Province） and 0. 29%（Guanxi Region）. CONCLUSION The established method can satisfy the requirements of European Pharmacopoeia in specificity,precision and accuracy of P. corylifolia L.

关 键 词：补骨脂 HPLC 补骨脂素 异补骨脂素 《欧洲药典》 

分 类 号：R284.1[医药卫生—中药学]