Mus81沉默对结肠癌HCT116细胞奥沙利铂敏感性的影响  

Effects of Mus81 gene silencing on oxaliplatin chemosensitivity of human colon cancer HCT116 cell

作　　者：常颖智[1] 吴帆[2,3] 曹杰[1] 谭卫民[1] 

机构地区：[1]广州市第一人民医院外科,510180 [2]广州市红十字会医院 [3]暨南大学医学院第四附属医院普通外科,510220

出　　处：《实用医学杂志》2015年第8期1219-1221,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:81000989);广州市珠江科技新星专项(编号:2011J2200008);广州市医药卫生科技项目(编号:20131A011006)

摘　　要：目的:观察Mus81基因沉默对人结肠癌细胞HCT116奥沙利铂敏感性的影响并分析其机制。方法:采用慢病毒介导的RNAi技术,构建Mus81基因沉默的HCT116细胞,MTT法检测奥沙利铂的IC50值。Annexin V-APC染色法检测奥沙利铂处理后的细胞凋亡率,并以实时定量PCR及Western blot检测p53、Bax及Bcl-2的表达水平。结果 :Mus81沉默的HCT116细胞对奥沙利铂的IC50值明显降低,该细胞经奥沙利铂处理后凋亡率上升了77.6%(P<0.01),p53和Bax的表达明显升高(P<0.01),Bcl-2表达则明显降低(P<0.01),导入Mus81基因则可降低该细胞凋亡率。结论 :Mus81基因沉默可提高HCT116细胞对奥沙利铂的敏感性,其机制可能是调控p53、Bax及Bcl-2的表达从而促进细胞凋亡。Objective To investigate the eitects of Mus81 gene silencing on the chemosensitivity of human colon cancer HCT116 cells to oxaliplatin and explore the underlying mechanisms. Methods lentiviral-mediated RNAi was employed to inhibit Mus81 gene in human colon cancer HCTll6 cells. MTF assay was employed to evaluate oxaliplatin chemosensitivity of HCTll6. Cell apoptosis rate was detected by Annexin V-APC staining and the expression levels of p53, while Bax and Bcl-2 in HCT116 cells were detected by Real-time PCR and Western blot. Results Oxaliplatin ICs0 of MusS1 depleted HCT116 cells was significantly decreased. After oxaliplatin treatment, the cell apoptosis rate of Mus81 silenced HCTll6 cells was increased by 77.6% （P 〈 0.05）, and the expression of p53 and Bax in these cells was obviously increased but the expression of Bcl-2 was significantly decreased （P 〈 0.01）. Introducing Mus81 gene in Mus81 depleted HCTll6 cells could reduce apoptosis rate. Conclusion Mus81 gene silencing can significantly improve oxliplatin chemosensitivity of HCT116 cells possibly by promoting cell apoptosis through regulating the expression of p53, Bax and Bcl-2.

关 键 词：结肠肿瘤 Mus81 奥沙利铂 化疗敏感性 凋亡 

分 类 号：R735.35[医药卫生—肿瘤]