A mimotope of Pre-S_2 region of surface antigen of viral hepatitis B screened by phage display  

作　　者：WU YU ZHANG, YING WAN, DAO GUO LI, YAN TANG, WEI ZHOU Institute of Immunology, PLA, The Third Medical University, Chongqing 400038, China Medical Center, General Hospital of Weifang, Weifang 26100, China 

出　　处：《Cell Research》2001年第3期203-208,共6页细胞研究（英文版）

基　　金：Grants39789010 and 39700132 from the Natural ScienceFoundat,ion of China and agrant from National Ed-u~ation Ministery und

摘　　要：To acquire the phage-displayed mimotopes which mimic the specificity of hepatitis B virus surface antigen (HBsAg), a random peptide library expressing linear peptide with 12 amino asids in length were used to screen with the serum from a hepatitis B virus infected patient in the recovery phase. After 3 rounds of biopanning, the positive phages were confirmed by competitive ELISA using HBsAg/P33. Two phagotopes were identified and one of them was confirmed as mimotope by competition experiment. Based on the mimotpe, a multiple antigenic peptide with four branches was synthesized by solid phase peptide synthesis. The antiginicity and specificity of the synthesized antigen was tested in BALB/c mice compared with the native epitope-based antigen. The results showed that the mimotope-based antigen could evoke higher titer of antibodies with the same specificity of the epitope-based antigen. Those findings indicate mimotopes can be used in antigen and vaccine design.

关 键 词：MIMOTOPE hepatitis B virus raccine ANTIBODY 

分 类 号：R373.21[医药卫生—病原生物学]