Blockage of IGF-1R signaling sensitizes urinary bladder cancer cells to mitomycin-mediated cytotoxicity  

作　　者：SunHZ WuSF 

机构地区：[1]DepartmentofBiologicalRegulation,WeizmannInstituteofScience,Rehovot76100,Israel. [2]StateKeyLaboratoryofDrugResearch,ShanghaiInstituteofMateriaMedica,ChineseAcademyofSciences,294Taiy

出　　处：《Cell Research》2001年第2期107-115,共9页细胞研究（英文版）

摘　　要：A major problem which is poorly understood in the management of bladder cancer is low sensitivity to chemotherapy and high recurrence after transurethral resection. Insulin-like growth factor 1 receptor (IGF-1R) signaling plays a very important role in progression, invasion and metastasis of bladder cancer cells. In this study, we investigated whether IGF-1R was involved in the growth stimulating activity and drug resistance of bladder cancer cells. The results showed: The mRNAs of IGF-1, IGF-2 and IGF-1R were strongly expressed in serum-free cultured T24 cell line, whereas normal urothelial cells did not express these factors/receptors or only in trace levels; T24 cell responded far better to growth stimulation by IGF-1 than did normal urothelial cells; blockage of IGF1R by antisense oligodeoxynucleotide (ODN) significantly inhibited the growth of T24 cell and enhanced sensitivity and apoptosis of T24 cells to mitomycin (MMC). These results suggested that blockage of IGF-IR signaling might potentially contribute to the treatment of bladder cancer cells which are insensitive to chemotherapy.

关 键 词：Antibiotics  Antineoplastic Apoptosis Autocrine Communication Bladder Neoplasms Carcinoma  Transitional Cell Cell Division CYTOTOXINS Drug Resistance  Neoplasm Gene Expression Regulation  Neoplastic Gene Targeting Humans Insulin-Like Growth Factor I Insulin-Like Growth Factor II Microscopy  Electron MITOMYCIN Oligodeoxyribonucleotides  Antisense Protein Synthesis Inhibitors RNA  Messenger Receptor  IGF Type 1 Signal Transduction Tumor Cells  Cultured 

分 类 号：R737.14[医药卫生—肿瘤] R969.4[医药卫生—临床医学]