Thansgenic peanut plants obtained by particle bombardment via somatic embryogenesis regeneration system  

作　　者：DengXY WeiYZ 

机构地区：[1]InstituteofPlantPhysiologyandEcology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademySciences,Shanghai200032,China [2]InstituteofPlantPhysiologyandEcology,ShanghaiInstitutesforBiologicalSciences,Ch

出　　处：《Cell Research》2001年第2期156-160,共5页细胞研究（英文版）

基　　金：the Natinnal Biotechnology Reseaxch Project of 863 High Technology, contract No. 101-01-01-02.

摘　　要：After pre-culture and treatment of osmosis, cotyledons of immature peanut (Arachis hypogaea L.) zygotic embryos were transformed via particle bombardment with a plasmid containing a chimeric hph gene conferring resistance to hygromycin and a chimeric intron-gus gene. Selection for hygromycin resistant calluses and somatic embryos was initiated at 10th d post-bombardment on medium containing 10-25 mg/L hygromycin. Under continuous selection, hygromycin resistant plantlets were regenerated from somatic embryos and were recovered from nearly 1.6% of the bombarded cotyledons. The presence and integration of foreign DNA in regenerated hygromycin resistant plants was confirmed by PCR (polymerase chain reaction) for the intron-gus gene and by Southern hybridization of the hph gene. GUS enzyme activity was detected in leaflets from transgenic plants but not from control, non-transformed plants. The production of transgenic plants are mainly based on a newly improved somatic embryogenesis regeneration system developed by us.

关 键 词：CINNAMATES Anti-Bacterial Agents Arachis hypogaea Cell Culture Techniques CHIMERA COTYLEDON Drug Resistance Gene Expression Regulation  Plant Genetic Engineering Hygromycin B Osmosis Plants  Genetically Modified Plasmids Regeneration Research Support  Non-U.S. Gov't Seeds Transformation  Genetic 

分 类 号：S565.203.5[农业科学—作物学]