应用SCID和NOD/SCID小鼠构建急性髓系白血病模型成瘤率的比较  

Comparison of the Tumor-forming Rate between the SCID and NOD/SCID Mice used to Set up Acute Myeloid Leukemia Model

作　　者：杨红[1] 刘建兴[2] 周泽平[1] 刘琳[1] 张铀[1] 

机构地区：[1]昆明医科大学第二附属医院血液科,云南昆明650101 [2]昆明医科大学,云南昆明650101

出　　处：《中国实验血液学杂志》2015年第2期328-334,共7页Journal of Experimental Hematology

基　　金：云南省应用基础研究计划项目-联合专项(2011FB198)

摘　　要：目的:比较应用SCID和NOD/SCID小鼠构建急性髓系白血病模型成瘤率的不同。方法:分别给SCID和NOD/SCID小鼠腹腔注射HL-60细胞,观察小鼠的一般情况、应用流式细胞术检测骨髓细胞CD33阳性率,病理学检查鉴定动物模型。结果:应用SCID小鼠构建急性髓系白血病模型成瘤率为30%,而应用NOD/SCID小鼠构建急性髓细胞白血病模型成瘤率为100%。结论:接种HL-60的NOD/SCID小鼠相对于SCID小鼠成瘤率高,小鼠发病率稳定,更适宜应用于白血病机制研究。Objective ：To compare the tumor-forming rate between the SCID and NOD/SCID mice to set up acute myeloid leukemia （AML） mouse model. Methods：The SCID and NOD/SCID mice were injected with HL-60 cells in to the abdominal cavity in order to contruct the AML mouse model. The gereral status of mice was observed, the positive rate of CD33 in bone marrow ceils was detected by flow cytometry, the mouse model was identified by pathologic examination. Results：The tumor-forming rate in constructed model using SCID mouse was 30%, while the tumor-forming rate in constructed model using NOD/SCID mouse was 100%. Conclusion：Compared with SCID mice, the tumor-forming rate in NOD/SCID mice injected with HL-60 cells is high, the incidence of AML is stable, suggesting that the NOD/SCID mouse model is more suitable to explore the pathogenesis of leukemia.

关 键 词：急性髓细胞白血病 SCID小鼠 NOD/SCID小鼠 

分 类 号：R733.71[医药卫生—肿瘤]