下调PPP2R5C表达对Jurkat细胞TAL1相关调控基因表达谱影响的初步分析  

Effects of Down-regulating PPP2R5C Expression on Expression Profile of TAL1-related Regulating Genes in Jurkat Cells

作　　者：陈宇[1,2,3] 刘思初[2] 杨力建[2] 陈少华[2] 张涛[2] 罗更新[2] 李扬秋[2,3] 

机构地区：[1]广东省医学科学院,广东省人民医院医学研究中心,广东广州510080 [2]暨南大学血液病研究所 [3]暨南大学再生医学教育部重点实验室,广东广州510632

出　　处：《中国实验血液学杂志》2015年第2期345-349,共5页Journal of Experimental Hematology

基　　金：广东省科技计划项目(2012B050600023);广东省高等学校科技创新重点项目(cxzd1013)

摘　　要：目的:前期研究显示,下调PPP2R5C表达能抑制T细胞白血病细胞株Jurkat细胞增殖。本研究利用基因芯片技术分析RNA干扰下调PPP2R5C表达对Jurkat细胞中TAL1通路相关基因的影响。方法:利用核转染技术将PPP2R5C-siRNA799转导Jurkat细胞,培养48 h后,提取细胞总RNA,纯化mRNA后逆转录制备目标序列,与Affymetrix基因表达谱芯片3'IVT杂交,以Affymetrix Gene Chip Scanner 3000扫描仪扫描得到原始图像数据,并应用Gene spring GX 11.0软件进行差异表达谱分析。结果:基因芯片分析的TAL1信号通路相关的26个基因全部发生了差异表达,其中上调表达的基因有15个,下调表达的基因有11个;明显上调表达的基因有GATA1、TCF4、XRCC6和TCF3,而明显下调表达的基因有SIN3A和RUNX1。结论:下调Jurkat细胞PPP2R5C基因表达,在一定程度上能抑制TAL1信号通路基因,从而抑制Jurkat细胞增殖。Objective： Based on our previous study showing the inhibition of lenkemia T cell proliferation by down- regulating PPP2R5C expression, this study was aimed to analyze the influence of down-regulating PPP2R5 expression via RNA interference on genes relatied with TALl signaling pathway by using gene chip technique. Methods： The PPP2R5C-siRNA799 was transduced into Jurkat cells by nucleofection, the total RNA was isolated from treated Jurkat cells after culture for 48 hours; the target sequences were prepared by revevse transcription after mRNA purification, and were hybridized with affymetrix gene expression profile chip 3＇ IVT. The original image data were collected using affymetrix gene chip scanner 3 000, and the gene expression profile was analyzed using gene spring GX 11.0 software. Results： The expression of all 26 genes related with TALl signaling pathway was changed, out of which the expression of 15 genes were up-regulated and the expression of 11 genes was down-regulated in PPP2R5C-siRNA 799-transfected Jurkat cells. The genes with significantly up-regulated expression were GATA1, TCF4, XRCC6 and TCF3, while the genes with significantly down-regulated expression were SIN3A and RUNX1. Conclusion： The down-regulation of PPP2R5C gene expression in Jurkat cells via RNA interference to a certain degree can inhibit TALl signaling pathway genes, thereby suppresses the proliferation of Jurkat cells.

关 键 词：PPP2R5C基因 TAL1基因 JURKAT细胞 

分 类 号：R733.7[医药卫生—肿瘤]