套细胞淋巴瘤患者β-catenin和P-GSK-3β表达的变化  

Expression Changes of β-catenin and P-GSK-3β in Patients with Mantle Cell Lymphoma

作　　者：何金水[1] 黄轶群[2] 翁剑鸣[3] 肖丽云[1] 翁开枝[1] 马旭东[2] 

机构地区：[1]福建医科大学附属漳州市医院儿科,福建漳州363000 [2]福建医科大学附属漳州市医院血液科,福建漳州363000 [3]福建医科大学附属漳州市医院病理科,福建漳州363000

出　　处：《中国实验血液学杂志》2015年第2期407-410,共4页Journal of Experimental Hematology

基　　金：福建省卫生厅青年科研课题(2012-2-114)

摘　　要：目的:通过检测经典Wnt信号通路中β-catenin和P-GSK-3β在套细胞淋巴瘤中的异常表达,探讨其与MCL发病的关系。方法:采用S-P法免疫组织化学染色技术检测β-catenin蛋白和P-GSK-3β蛋白在套细胞淋巴瘤和增生性淋巴结炎中的表达差异。结果:套细胞淋巴瘤组的β-catenin蛋白异常表达为73.33%,明显高于淋巴结反应性增生组中的异常表达6.7%(P<0.05);P-GSK-3β在套细胞淋巴瘤组阳性率为66.67%,显著高于其在淋巴结反应性增生组中的表达16.67%(P<0.05)。Spearman相关分析发现,两者存在明显的正相关(r=0.852,P<0.01)。结论:套细胞淋巴瘤存在β-catenin和P-GSK-3β蛋白的异常高表达。Objective： This study was purposed to detect the expressions of β -catenin and P-GSK-3 β in Wnt signaling pathway of patients with mantle cell lymphoma（ MCL）, and investigate its relationship with the pathogenesis of MCL. Methods： The expression levels of 13 -catenin protein and P-GSK-3 protein in mantle cell lymphoma and hyperplastic lymphadenitis were detected by using anti-β-catenin, P-GSK-3β polyclonal antibody and S-P staining technique. Results： The abnormal expression of β-catenin protein（73. 33% ） in mantle cell lymphoma group was significantly higher than that （6.7%） in reactive lymph node hyperplasia group （ P 〈 0.05 ） ; and the positive rate of P- GSK-3 β（66. 67% ） in mantle cell lymphoma group was significantly higher than that （ 16.67% ） in reactive hyperplasia of lymph node group （ P 〈 0.05 ）. Spearman correlation analysis showed that there was obvious positive correlation （R = 0. 852, P 〈 0. 01 ）. Conclusion： The abnormal high expressions of β-catenin and P-GSK-3 β protein have been confirmed to appeare in mantle cell lymphoma.

关 键 词：套细胞淋巴瘤 Β-连环素 P-GSK-3β GSK-3Β 

分 类 号：R733.1[医药卫生—肿瘤]