虾青素对红细胞氧化应激及胞膜过氧化损伤的影响  

Effect of Astaxanthin on Oxidative Stress of Red Blood Cells and Peroxidation Damage of Membrane

作　　者：王素玲[1] 何路军[1] 何天博[2] 韩卫[1] 王切[3] 

机构地区：[1]河北省血液中心检验科,河北石家庄050071 [2]河北医科大学第四医院检验科,河北石家庄050000 [3]河北医科大学解剖教研室,河北石家庄050017

出　　处：《中国实验血液学杂志》2015年第2期552-556,共5页Journal of Experimental Hematology

基　　金：河北省科技支撑计划项目(13277717D)资助

摘　　要：目的:探讨抗氧化剂虾青素(Astaxanthin,ASTA)对储存悬浮红细胞内外氧化应激水平的影响和对胞膜的保护作用。方法:采集志愿者血液,制备去白悬浮红细胞,将其随机分为4组:A组、B组、C组、D组。在B、C、D3组悬浮红细胞的MAP保存液内加入抗氧化剂ASTA,使其终浓度分别为5、10和20μmol/L,A组作为对照组只加入ASTA的溶解液DMSO。悬浮红细胞于2℃-6℃冰箱内保存。于保存第7、14、28、42天,采用荧光酶标仪测定红细胞内活性氧族(ROS)含量;硫代巴比妥酸比色法测定红细胞内丙二醛(MDA)含量;分光光度法测定细胞外过氧化氢(H2O2)含量,全自动血细胞分析仪测定平均红细胞体积(MCV);化学比色法测定细胞外游离血红蛋白含量(FHb)。结果:在红细胞储存期间B、C、D组悬浮红细胞内ROS、MDA含量、细胞外FHb和H2O2含量均低于对照组;保存第7天和14天时B、C、D组的MCV与对照组无统计学差异;保存第28天和42天时B、C、D组的MCV低于对照组。结论:ASTA可以降低储存悬浮红细胞内外的氧化应激水平,减轻细胞膜的过氧化损伤。Objective：To explore the effect of astaxanthin （ASTA） on oxidative stress of intra - and extra - red blood cells during stored period and the protective function for cell membrane. Methods：The blood of volunteers was collected to prepare suspended red blood cells without leukocytes. Then the red blood cells were randomly divided into group A, group B, group C and group D. The ASTA was added into MAP preservation solution of group B, group C and group D, the final concentration of ASTA was 5, 10 and 20 μmol/L respectively. Group A was used as control group, in which only the dissolved liquid DMSO of ASTA was added. The red blood cells were stored in refrigerator at 2 ℃ - 6 ℃. On day 7, 14, 28 and day 42 of storage, the content of reactive oxygen species （ROS） in red blood cells was detected by fluorescence microplate reader. The content of malondialdehyde （MDA） was detected with TBA method. The content of hydrogen peroxide （ H2O5 ） outside cell was detected with spectrophotometric method. The mean corpuscular volume（MCV） was detected with blood cell analyzer. The content of free hemoglobin（FHb） was detected with chemical colorimetry. Results：The ROS, MDA, FHb and H202 levels in B, C and D groups were lower than those in control group during the stored period. On day 7 and 14 of storage, among group B, group C, group D and group A, the MCV showed no difference in comparison with control group. On day 28 and 42 of storage, the MCV in B, C and D groups was lower than that in control group. Conclusion：The ASTA can reduce the oxidative stress level of stored red blood cells inside and outside, relieve the peroxidation damage of cell membrane.

关 键 词：虾青素 悬浮红细胞 氧化应激 活性氧族 游离血红蛋白 

分 类 号：R331.141[医药卫生—人体生理学]