Mn、Zn单一及复合污染对水蓼氮素代谢的影响  

Effects of Mn/Zn Single and Combined Pollution on the Nitrogen Metabolism in Polygonum Hydropiper L.

作　　者：刘华[1] 刘可慧[2] 周振明[1,3] 苏银萍[1] 陈孟林[1,3] 陈朝述[1,3] 于方明[1,3] 

机构地区：[1]广西师范大学环境与资源学院,广西桂林541004 [2]桂林电子科技大学生命与环境学院,广西桂林541004 [3]广西环境污染控制与技术重点实验室,广西桂林541004

出　　处：《生态环境学报》2015年第3期517-521,共5页Ecology and Environmental Sciences

基　　金：国家自然科学基金项目(41161057);广西科学研究与技术开发项目重大专项计划(桂科重1298002-6);广西教育厅项目(2013HZ003);广西自然科学基金项目(2014GXNSFAA118303);广西科学研究与技术开发项目(桂科转14122008-2)

摘　　要：采用水培的方法,研究了Mn、Zn单一及复合污染对锰超富集植物水蓼（Polygonum hydropiper L.）生长、氮代谢关键酶（谷氨酰胺合酶（GOGAT）、谷氨酸合成酶（GS）、硝酸还原酶（NR）、谷氨酸脱氢酶（GDH））活性以及硝态氮（NO3-）、铵态氮（NH4＋）、可溶性蛋白质、游离脯氨酸、叶绿素a、叶绿素b和叶绿素a＋b含量,水蓼对Mn、Zn的吸收和转移系数的影响。研究结果表明,水蓼根、茎、叶中Mn、Zn含量,随着Mn、Zn处理浓度的增加而增加,且水蓼对Zn和Mn的转移系数分别维持在1.0-1.8和1.0-11.9,表明Zn对水蓼体内Mn的转移影响较为明显;Mn/Zn单一及复合处理显著降低了水蓼叶绿素a、叶绿素b和叶绿素a＋b的含量（P〈0.05）,但是叶绿素a/b较对照组显著提高（P〈0.05）,Mn/Zn复合处理抑制了根系生长及株重,T8时,根长仅为对照组的64.93%,而T9时,株重仅为对照组的65.45%;单Zn及Mn/Zn复合处理均降低了水蓼根系中NO3-的含量,以及抑制NR活性,T5时,叶片和根中NR活性分别是对照组的44.36%和34.41%,但提高了叶片与根系中NH4＋的含量,T9时叶片中的NH4＋含量是对照组的2.35倍,T8时根中的NH4＋含量是对照组的1.58倍,以及增加了GS活性,T9时,叶和根中GS活性分别使对照组的1.32和1.57倍,Zn及Mn、Zn复合处理对水蓼叶片与根系中可溶性蛋白质含量无显著影响（P〉0.05）,表明GS活性以及GDH活性提高在消除NH4＋胁迫过程中起重要作用。Hydroponic culture was conducted to study the effect of Mn and Zn combined single and pollution on the growth, heavy metals of Mn and Zn accumulation and transfer factor（TF）, nitrogen metabolism in Mn-hyperaccumulatorPolygonum hydropiper L.. The enzymatic activies of nitrogen metabolism including nitrate reductase （NR）, glutamine synthetase （GS）, glutamineate synthase （GOGAT） glutamate dehydrogenase （GDH） as well as the concentrations of chlorophyll a, b a＋b, a/b, NO3-, NH4＋, free proline and soluble protein inPolygonum hydropiper L. was determined. Resalts showed that Mn and Zn concentrations of roots, stems and leaves of thePolygonum hydropiper L. , with the increase Mn and Zn control concentration increased,Polygonum hydropiper L. vary 1.0 from 11.9 of Mn and vary 1.0 from 1.8 of Zn of transfer factor. It indicated that Zn had affect on transfer of Mn. Mn/Zn treatment singinficantly reduced contents of chlorophyll a, b and a＋b（P〈0.05）, but the chlorophyll a/b significantly increased than the control group （P〈0.05）. Mn/Zn treatment restrained growth of roots and biomass, when T8, root is only 64.93% and T9, biomass is only 65.45% of that in the control group. What single Zn and Mn/Zn combined pollution reduced the contents of NO3- and NR activities, when T5, NR activity in leaves and roots, respectively is 44.36% and 34.41% in the control group; but increased the contents of NH4＋, T9, leaf of NH4＋ content is 2.35 times that of the control group and T8 root of NH4＋content is 1.58 times that of the control group; GS activities significantly increased than the control group （P〈0.05） of roots and leaves, GS activities are 1.32 and 1.57 times that of the control group of leaf and ront when T9; had no singinficantly efected on soluble protein of roots and leaves ofPolygonum hydropiper L.. It indicated that play an important role in the process of elimination NH4＋ stress.

关 键 词：Mn/Zn复合污染 水蓼 氮素代谢 

分 类 号：X171.5[环境科学与工程—环境科学]