白藜芦醇甙对慢性酒精中毒大鼠学习记忆及海马Cdk5激酶活性的影响  

Effects of polydatin on learning and memory and Cdk5 kinase activity in the hippocampus of rats with chronic alcoholism

作　　者：李新娟[1,2] 张艳[1,2] 徐春阳[1,3] 李爽[1,2] 杜爱林[1,2] 张利彬[1,2] 张瑞岭[1,4] 

机构地区：[1]新乡医学院河南省生物精神病学重点实验室 [2]生理学教研室 [3]免疫学教研室,新乡453003 [4]新乡医学院二附院,河南新乡453003

出　　处：《中国应用生理学杂志》2015年第2期117-120,共4页Chinese Journal of Applied Physiology

基　　金：新乡医学院科研项目培育基金(2013ZD112);河南省生物精神病学重点实验室开放课题(ZDSYS2011003);河南省科技厅基础研究项目(142300410026)

摘　　要：目的:研究白藜芦醇甙对慢性酒精中毒大鼠学习记忆及海马细胞周期依赖性蛋白激酶5(Cdk5)活性的影响。方法:40只大鼠随机分为4组(n=10):对照组、酒精中毒模型组、酒精中毒+白藜芦醇甙低剂量组、酒精中毒+白藜芦醇甙高剂量组。以灌胃法(每天3.0 g/kg酒精)建立慢性酒精中毒大鼠模型。戒断评分法观察戒断症状;Morris水迷宫测试学习记忆成绩;免疫荧光法检测大鼠海马区Cdk5蛋白表达;液闪法检测海马组织Cdk5激酶活性。结果:酒精中毒模型组戒断评分、水迷宫测试结果、Cdk5激酶活性,均明显高于对照组(P<0.05);白藜芦醇甙高剂量组戒断评分、水迷宫测试结果,均明显低于酒精中毒模型组(P<0.05);白藜芦醇甙高低剂量组Cdk5激酶活性均明显低于酒精中毒模型组(P<0.05);酒精中毒模型组大鼠海马区Cdk5阳性细胞较对照组明显增加(P<0.05),给予白藜芦醇甙干预后Cdk5阳性细胞数量较酒精中毒模型组减少(P<0.05)。结论:白藜芦醇甙能够缓解酒精中毒所致的记忆损伤,这种作用可能与下调Cdk5激酶活性有关。Objective： To observe the effects of polydatin on learning and memory and cyclin-dependent kinase 5 （CdkS） kinase activity in the hippocampus of rats with chronic alcoholism. Methods： Forty rats were randomly divided into 4 groups ： control group, chronic alcoholism group, low and high polydatin group. The rat chronic alcoholism model was established by ethanol 3.0 g/（ kg· d） （ intragastric administration）. The abstinence scoring was used to evaluate the rats withdrawal symptoms; cognitive function was measured by Morris water maze experiment; Cdk5 protein expression in the hippocampus was detected by immunofluorescence; Cdk5 kinase activity in the hippocampus was detected by liquid scintillation counting method, Results： The abstinence score, escape latency, Cdk5 kinase activity in chronic alcoholism group rats were significantly higher than those of control group （ P 〈 0.05）. The abstinence score, escape latency in high polydatin group rats were significantly lower than those of chronic alcoholism group （ P 〈 0. 05） ; Cdk5 kinase activity in high and low polydatin group rots was significantly lower than that of chronic alcoholism group（ P 〈 0. 05） ; immunofluorescence showed that the Cdk5 positive cells of chronic alcoholism group were signifi- cantly increased compared with control group （ P 〈 0. 05）, and the Cdk5 positive cells of polydatin groups were significantly decreased com- pared with chronic alcoholism group （ P 〈 0.05）. Conclusion： Polydatin-reduced the chronic alcoholism damage may interrelate with regulation of Cdk5 kinase activity.

关 键 词：白藜芦醇甙 慢性酒精中毒 细胞周期依赖性蛋白激酶5 学习记忆 

分 类 号：R742[医药卫生—神经病学与精神病学]