角膜内皮细胞培养的研究进展  

Research advances in corneal endothelial cells culture

作　　者：吴敏[1,2] 胡竹林[1,2] 

机构地区：[1]昆明医科大学第四附属医院眼科 [2]云南省第二人民医院眼科,云南省昆明市650021

出　　处：《眼科新进展》2015年第5期485-488,共4页Recent Advances in Ophthalmology

基　　金：云南省应用基础研究项目(昆医联合专项)(编号:2013FB109);云南省教育厅科学研究基金项目(编号:2011C079);云南省卫生科技计划项目(编号:2012WS0109);云南省高层次卫生技术人才培养专项经费资助~~

摘　　要：角膜内皮功能失代偿引起的失明可通过角膜移植来治疗。人们通过体外培养角膜内皮细胞并使其增殖,进行角膜内皮细胞移植和角膜组织工程学研究。目前常用的分离角膜内皮细胞的方法是从供体上撕除后弹力层和角膜内皮层,利用酶消化作用使角膜内皮细胞分离。常用的培养基为基础培养基加各种生长因子。这些生长因子在一定程度上促进细胞增殖,但培养的角膜内皮细胞长期传代后仍难以维持正常的细胞形态和功能。基因转染技术建立了永生化角膜内皮细胞系,有关细胞周期、信号通路和离子通道的研究成果也促进了角膜内皮细胞体外培养的研究进展。本文综述了角膜内皮细胞体外培养各个环节的最近进展。Corneal endothelial dysfunction can be treated by keratoplasty. Because of extremely limited sources of donor cornea, cultured corneal endothelial cells （CEC） in vitro can be proliferated and applied in CEC transplantation and researches of tissue engineering cornea which brings new hope to corneal endothelial dysfunction. Current- ly, the most commonly used method to isolate CEC is stripping Descemet＇ s membrane with endothelium from donor cornea and enzyme digestion. Commonly used culture me- dium consists of basal medium and different growth factors which can promote the pro- liferation of CEC to some extent. Cultured CEC after several passages are difficult to re- main the normal phenotypes and cell morphology. Recently, the immortalizing cell line was established by gene transfection. The advancement in cell cycle,signal pathway and ion channel of CEC will promote the progression of cell culture in vitro. This article re- views the research advances in corneal endothelial cells culture in vitro.

关 键 词：角膜内皮细胞 细胞培养 体外 

分 类 号：R774.120.2[医药卫生—眼科]