唐古特大黄多糖组分1对大鼠急性放射性肠损伤的保护作用  

Protective role of Rheum tanguticum polysaccharide 1 on the radiation-induced acute intestinal mucosal injury

作　　者：刘琳娜[1] 张甜[1] 石磊[1] 张志培[1] 李诗草[1] 关波[1] 张文娟[1] 

机构地区：[1]第四军医大学唐都医院药剂科,陕西西安710038

出　　处：《胃肠病学和肝病学杂志》2015年第4期414-417,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然科学基金(81073135)

摘　　要：目的探讨唐古特大黄多糖组分1(Rheum tanguticum polysaccharides,RTP1)对辐射所致大鼠急性肠黏膜损伤的保护作用。方法 RTP1灌胃给药(剂量200、400、800 mg/kg)7 d后,除正常组大鼠外,其余各组均接受10.0 Gy/只一次性全腹均匀X射线照射1次,3 d后处死动物,观察小肠黏膜病理形态改变,测定肠黏膜屏障功能、小肠组织氧化还原酶活性及血浆内毒素水平。结果RTP1预处理后可以改善肠黏膜损伤,升高SOD活性及GSH含量,降低MDA水平,抑制血浆中DAO、D-乳酸及内毒素水平,与IC组比较,差异具有统计学意义(P<0.05)。结论 RTP1通过减轻肠黏膜屏障功能损害,增强抗氧化能力保护辐射所致肠黏膜损伤。Objective To investigate the protective role of Rheum tanguticum polysaccharides 1 （ RTP1 ） on the ra-diation-induced acute intestinal mucosal injury in rats .Methods Rats in RTP1 group were treated with 200 , 400 or 800 mg/kg RTP1 by intragastric administration for 7 days.At the 7th day, rats in IC group and RTP1 groups received a single dose of 10 .0 Gy for the abdomianl regions .At the 4 th day of radiation , the small intestinal samples were collected for pathology analysis and levels of SOD , MDA, GSH were also detected .The levels of DAO , D-lactate and endotoxin in blood plasma were measured .Results Compared with the IC group , the mucosa structure of small intestine in the RTP1 group was greatly improved .RTP1 pretreatment decreased the levels of MDA , the plasma levels of DAO D-lactate and endotoxin （P〈0.05）;enhanced the activity of SOD and the level of GSH （P〈0.05）.Conclusion RTP1 can promote the recovery of intestinal mucosa damage , which may be related to its inhibition of intestinal epithelial injury and intracellular reactive oxygen species production caused by radiation .

关 键 词：唐古特大黄多糖 辐射损伤 放射性肠炎 肠黏膜屏障 

分 类 号：R574[医药卫生—消化系统]