Structural components of the nuclear body in nuclei of Allium cepa cells  

作　　者：TAO WEI, CHANG HUI YAN, TAO CAI, SHUI HAO, ZHONG HE ZHAI (College of Life Sciences, Peking University, Beijing 100871, China) (Institute of Genetics and Cytology, Northeast Normal University, Changchun Jilin 130024, China) 

出　　处：《Cell Research》2001年第1期68-73,共6页细胞研究（英文版）

摘　　要：Nuclear bodies have long been noted in interphase nuclei of plant cells, but their structural component, origin and function are still unclear by now. The present work showed in onion cells the nuclear bodies appeared as a spherical structure about 0.3 to 0.8 microm in diameter. They possibly were formed in nucleolus and subsequently released, and entered into nucleoplasm. Observation through cytochemical staining method at the ultrastructural level confirmed that nuclear bodies consisted of ribonucleoproteins (RNPs) and silver-stainable proteins. Immunocytochemical results revealed that nuclear bodies contained no DNA and ribosomal gene transcription factor (UBF). Based on these data, we suggested that nuclear bodies are not related to the ribosome or other gene transcription activities, instead they may act as subnuclear structures for RNPs transport from nucleolus to cytoplasm, and may also be involved in splicing of pre-mRNAs.

关 键 词：Pol1 Transcription Initiation Complex Proteins Cell Nucleolus Cell Nucleus DNA DNA-Binding Proteins INTERPHASE Microscopy  Electron Onions Plant Components RNA  Messenger Research Support  Non-U.S. Gov't RIBONUCLEOPROTEINS Silver Staining Transcription Factors 

分 类 号：Q2[生物学—细胞生物学]