缺血预处理对局灶性脑缺血再灌注大鼠缺血侧海马CA1区低氧诱导因子-1α及存活素表达的影响  

Effects of ischemic preconditioning on the expression of hypoxia-inducible factor-1α and survivin at ischemia bilateral hippocampal CA1 area in rats with focal cerebral ischemia reperfusion

作　　者：王红霞[1,2] 李世英[1] 李峥[3] 张晋霞[1] 贺永贵[1] 余红[4] 孙妍[5] 刘斌[1] 

机构地区：[1]河北联合大学附属医院神经内一科,唐山063000 [2]中国水利水电第十三工程局医院神经内科 [3]唐山玉田县医院神经内科 [4]河北联合大学基础医学院药理教研室 [5]中国人民解放军第255医院神经内科

出　　处：《临床神经病学杂志》2015年第2期121-124,共4页Journal of Clinical Neurology

基　　金：河北省2013年医学科学研究重点课题计划(20130382)

摘　　要：目的探讨缺血预处理对局灶性脑缺血再灌注大鼠缺血侧海马CA1区低氧诱导因子-1α(HIF-1α)及存活素表达的影响。方法健康雄性SD大鼠130只随机分为假手术组(SO组,n=10)、局灶性脑缺血再灌注组(MCAO组,n=60)、缺血预处理组(BIP组,n=60),MCAO组和BIP组又分别分为再灌注2 h、6 h、12h、24 h、48 h、72 h 6个亚组,每亚组10只大鼠。采用线栓法制备大鼠脑缺血再灌注模型。BIP组在缺血前24h给予10 min的预缺血。采用免疫组化染色法检测HIF-1α、存活素的阳性细胞数。采用Western blot法检测HIF-1α、存活素的蛋白相对含量。结果与SO组比较,MCAO组和BIP组各时间点亚组大鼠缺血侧海马CA1区HIF-1α及存活素的阳性细胞数及蛋白相对含量均明显升高(均P<0.05)。与MCAO组比较,BIP组各时间点亚组大鼠缺血侧海马CA1区HIF-1α及存活素的阳性细胞数及蛋白相对含量均明显升高(均P<0.05)。在MCAO组和BIP组中,大鼠缺血侧海马CA1区HIF-1α及存活素的阳性细胞数及蛋白相对含量均在再灌注24 h时达到最高峰(均P<0.05)。结论缺血预处理能够诱导局灶性脑缺血再灌注大鼠缺血侧海马CA1区HIF-1α、存活素表达上调,这或许与脑缺血耐受的产生机制有关。Objective To observe the effects of ischemic preconditioning on the expression of hypoxia-inducible factor-1α（ HIF-1α） and survivin at ischemia bilateral hippocampal CA1 area in rats with focal cerebral ischemia reperfusion.Methods One hundred and thirty healthy male SD rats were randomly divided into sham operation group （ SO group, n =10 ） , focal cerebral ischemia reperfusion group （ MCAO group, n =60 ） and ischemia preconditioning group （ BIP group, n=60） .The MCAO group and BIP group were further divided into reperfusion 2 h, 6 h, 12 h, 24 h, 48 h and 72 h 6 subgroups, respectively, with 10 rats in each subgroup.Sutured method was used to made focal cerebral ischemia reperfusion model rats.The BIP group received ischemic preconditioning with 10 min at 24 h before cerebral ischemia.The numbers of positive cells of HIF-1αand survivin were detected by immunohistochemistry.The relative contents of protein of HIF-1αand survivin were detected by Western blot method.Results Compared with the SO group, the numbers of positive cells and the relative contents of protein of HIF-1α, survivin at ischemia bilateral hippocampal CA1 area in subgroups at each time point of MCAO group and BIP group were significantly increased （ all P〈0.05 ） .Compared with the MCAO group, the numbers of positive cells and the relative contents of protein of HIF-1α, survivin at ischemia bilateral hippocampal CA1 area in subgroups at each time point of BIP group were significantly increased （ all P〈0.05） .In the MCAO group and BIP group, the numbers of positive cells and the relative contents of protein of HIF-1α, survivin at ischemia bilateral hippocampal CA1 area were all arrived the peak at reperfusion 24 h moment （ all P〈0.05 ） .Conclusions Ischemic preconditioning can increase the expression of HIF-1α, survivin at ischemia bilateral hippocampal CA1 area in rats with focal cerebral ischemia reperfusion.It may be related to the mechanism of cerebral ischemia tolerance.

关 键 词：缺血预处理 脑缺血再灌注 低氧诱导因子-1Α 存活素 脑缺血耐受 

分 类 号：R743.3[医药卫生—神经病学与精神病学]