促性腺激素释放激素在雌二醇诱导的大鼠青春期启动提前中的作用  

Role of gonadotropin-releasing hormone on advancing rat puberty onset induced by estradiol

作　　者：华辰凤 杨嵘[1] 刘羽欣[1] 黄丽娜[1] 张丽[1] 张添光[1] 田冬冬[1] 赵君[1] 赵增明[1] 王以美[1] 彭双清[1] 

机构地区：[1]军事医学科学院疾病预防控制研究所毒理学评价研究中心,北京100071

出　　处：《中国药理学与毒理学杂志》2015年第2期260-264,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金(81172699)~~

摘　　要：目的探讨促性腺激素释放激素(Gn RH)在雌二醇(E2)诱导大鼠青春期启动提前中的作用。方法 18日龄雌性SD大鼠分别经口给予玉米油和E250μg·kg-1,连续给药5 d。观察大鼠阴道开口时间及卵巢组织变化;Western蛋白印迹法检测下丘脑的Gn RH、G蛋白偶联受体54(GPR54)和磷脂酶C(PLC)蛋白表达水平。结果与玉米油对照组相比,E2组大鼠阴道开口时间显著提前约12.2 d(P<0.01)。卵巢组织切片可见黄体,且下丘脑Gn RH,GPR54和PLC蛋白表达水平分别增加47%,55%和56%(P<0.05)。结论 E2诱发的大鼠青春期启动时间提前可能与调节下丘脑Gn RH,GPR54和PLC表达相关。OBJECTIVE To investigate the role of gonadotropin-releasing hormone （GnRH） in es-tradiol（E2 ） induced advance of puberty onset in rats. METHODS Postnatal day 18 SD rats were given a daily intragastric administration of corn oil or E2（50 μg.kg-1 ） for consecutive 5 d. The day of vaginal opening （VO）, pathological changes in ovary and protein expression levels of GnRH, G protein-coupled receptor 54 （ GPR54） and phospholipase C （ PLC） in hypothalamus were observed. RESULTS As compared to corn oil controll group, VO was advanced by about 12.2 d, corpus luteum was observed in the ovary section, and the protein expression levels of GnRH,GPR54 and PLC in hypothalamus were significantly increased by 47%, 55% and 56% in E2 group, respectively. CONCLUSION E2 induced onset of puberty advance may be closely related to regulation of the expression of GnRH, GPR54 and PLC in hypothalamus.

关 键 词：雌二醇 青春 早熟 促性腺激素释放激素 

分 类 号：R725.8[医药卫生—儿科]