miR-375对人结肠癌细胞株HCT116活性、细胞周期及凋亡的影响  

Effect of miR-375 on viability,cell cycle and apoptosis of colon cancer HCT116 cells

作　　者：刘宝龙[1,2] 吴斌文[2] 黄素军 李东风[2] 

机构地区：[1]南方医科大学,广东广州510515 [2]广东省人民医院,广东省老年医学研究所,广东省医学科学院,广东广州510080 [3]广州市红十字会医院,广东广州510220

出　　处：《中国病理生理杂志》2015年第4期609-614,共6页Chinese Journal of Pathophysiology

基　　金：广东省科技计划(No.2011B031800001)

摘　　要：目的:探讨微小RNA-375(microRNA-375,miR-375)对人结肠癌细胞HCT116活性、细胞周期及凋亡的影响。方法:Real-time PCR检测不同结直肠癌细胞中miR-375的表达情况。脂质体转染法将miR-375模拟物(mimics)转入HCT116细胞,用real-time PCR法检测miR-375及AEG-1 mRNA的表达情况;MTT法检测细胞活性的改变情况;流式细胞技术检测miR-375对细胞凋亡及细胞周期的影响。结果:Real-time PCR结果显示HCT116在4个结直肠癌细胞株中miR-375的表达量最低;miR-375 mimics组中miR-375表达量较对照组明显上调;miR-375高表达可以显著抑制AEG-1 mRNA的表达水平。miR-375 mimics组细胞活性明显受到抑制,同时细胞凋亡率明显增加,G1期所占细胞数增加,而S期所占细胞数减少。结论:miR-375可以抑制结肠癌HCT116细胞的活性,介导细胞周期阻滞并促进其凋亡。miR-375作为一种抑癌因子,在结肠癌中可能通过抑制AEG-1发挥抑癌作用。AIM：To investigate the effect of microRNA-375 （ miR-375） on the viability, cell cycle and apop-tosis of HCT116 cells.METHODS： The expression of miR-375 in different colorectal cancer cell lines was detected by real-time PCR.The miR-375 mimics was transfected into HCT116 cells by LipofectamineTM 2000.The mRNA expression of miR-375 and AEG-1 was detected by real-time PCR.The HCT116 cell viability was detected by MTT assay.The changes of apoptosis and cell cycle distribution were analyzed by flow cytometry.RESULTS：Real-time PCR showed that miR-375 expression was the lowest in HCT116 among 4 colorectal cancer cell lines.The expression level of miR-375 significantly in-creased in miR-375 mimics group compared with that in the negative control group.The high expression level of miR-375 significantly inhibited the mRNA expression of AEG-1.After transfection with miR-375 mimics, the cell viability was in-hibited, the apoptotic rate was increased, the proportion of G1-stage cells was increased, and the proportion of S-stage cells was decreased.CONCLUSION：miR-375 inhibits the viability, mediates the cell cycle arrest and promotes the apoptosis of colon cancer HCT116 cells.miR-375 may act as a tumor suppressor in colorectal cancer by inhibiting AEG-1.

关 键 词：结直肠癌 微小RNA-375 细胞活性 细胞周期 细胞凋亡 

分 类 号：R735.3[医药卫生—肿瘤] R730.23[医药卫生—临床医学]