静脉注射IL-19重组质粒对大鼠实验性自身免疫性心肌炎的治疗作用  

Effect of intravenous injecting plasmid encoding interleukin-19-IgG on experimental autoimmune myocarditis in rats

作　　者：常贺[1,2] 赵法允 王焱[1,2] 李刚 张乐 邹军[1] 

机构地区：[1]厦门大学医学院,福建厦门361102 [2]厦门市心脏中心,福建厦门361004

出　　处：《中国病理生理杂志》2015年第4期744-749,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81270294);福建省卫生系统中青年骨干人才项目(No.2013-ZQN-ZD-37);福建省自然科学基金资助项目(No.2012J01415)

摘　　要：目的:评估静脉注射白细胞介素19(interleukin-19,IL-19)重组质粒对大鼠实验性自身免疫性心肌炎(experimental autoimmune myocarditis,EAM)的治疗作用。方法:将猪心室肌球蛋白与等体积完全弗氏免疫佐剂充分混匀后,双足皮下注射制作大鼠EAM模型,免疫后第6天应用静脉注射方法将IL-19重组质粒导入体内,第17天行心脏超声检查后处死大鼠,检测心脏重量与体重比值、病理学评估、心肌炎面积率;实时荧光定量PCR检测心衰标记物心房钠尿肽(atrial natriuretic peptide,ANP)、脑钠尿肽(brain natriuretic peptide,BNP)的表达水平,进一步检测心肌相关炎症因子IL-18、IL-1β、IL-12p35和IFN-γ的表达水平。结果:IL-19重组质粒导入组的大鼠心功能得到显著改善;心脏重量体重比、心肌炎面积率、ANP、BNP的表达均较模型组明显下降;相关炎症细胞因子的表达也显著降低。结论:应用静脉注射法进行IL-19重组质粒体内导入治疗,明显抑制大鼠实验性自身免疫性心肌炎的炎症反应,减轻了心肌损伤从而改善心功能。AIM：To evaluate the effect of intravenous injecting plasmid encoding interleukin-19-IgG on exper-imental autoimmune myocarditis （ EAM） in rats.METHODS：Cardiac myosin was emulsified with equal volume of com-plete Freund’ s adjuvant.The animal model of EAM was established by injecting with the preparation in both footpads of the Lewis rats.The rats were intravenously injected with the plasmid encoding IL-19-IgG on day 6.Echocardiography was per-formed before the rats were sacrificed on day 17.The effect of IL-19-IgG plasmid injection was evaluated by measuring the heart weight/body weight, myocarditis area, relative expression levels of atrial natriuretic peptide （ ANP） and brain natri-uretic peptide （BNP） in the hearts.The mRNA expression levels of related cytokines including IL-18, IL-1β, IL-12p35 and IFN-γwere detected.RESULTS：The rats in model group showed significant myocardial damage and a decrease in the left ventricular functions.The rats in the treatment group injected with IL-19-IgG plasmid showed an improvement of the cardiac functions.The ratio of heart weight/body weight, the area of myocarditis and the mRNA levels of ANP and BNP were significantly lower in IL-19-IgG treatment group than those in model group.The mRNA levels of IL-18, IL-1β, IL-12p35 and IFN-γwere also significantly decreased in IL-19-IgG treatment group.CONCLUSION：Intravenous injection of plasmid encoding IL-19-IgG effectively prevents the development of the left ventricular remodeling and myocardial damage in EAM rats.

关 键 词：白细胞介素19 自身免疫性心肌炎 

分 类 号：R363.2[医药卫生—病理学]