原花青素对小鼠脑缺血再灌注损伤的神经保护机制  

The Protective Effect of Procyanidins on Cerebral Ischemia Reperfusion Injury in Mice

作　　者：张文艳[1,2,3] 孙宝飞[1] 余资江[1] 余彦[1] 肖朝伦[1] 罗时鹏[1] 令狐艳[1] 杨丹[1] 

机构地区：[1]贵阳医学院解剖学教研室,贵州贵阳550004 [2]贵阳医学院附院呼吸内科,贵州贵阳550004 [3]贵阳医学院

出　　处：《贵阳医学院学报》2015年第4期352-355,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金(NO81060108)；贵州省科技厅社会发展攻关项目[黔科合SY字(2012 )3144号]

摘　　要：目的:探讨原花青素对小鼠脑缺血再灌注损伤的神经保护机制。方法:120只昆明小鼠随机分成正常对照组、假手术组、14 d模型组、14 d治疗组、28 d模型组、28 d治疗组;采用小鼠双侧颈总动脉结扎缺血15 min、再灌注15 min,反复3次构建脑缺血再灌注损伤模型;各治疗组在处死前7 d连续给予松树皮提取物灌胃治疗,其余各组灌注等量生理盐水,小鼠处死后取各组海马组织用ELISA法测定肿瘤坏死因子-α(TNF-α)及白介素-6(IL-6)的表达,westen blot法检测海马内核因子-κB(NF-κB)的表达。结果:各治疗组脑组织TNF-α、IL-6及NF-κB蛋白表达水平与同时点模型组比较均明显降低,差异有统计学意义(P<0.05)。结论:原花青素对脑缺血再灌注损伤小鼠神经保护机制可能是通过降低NF-κB、TNF-α以及IL-6表达而实现。Objective：To discuss the protective effect and possible mechanism of procyanidins on cerebral ischemia reperfusion injury in mice.Methods：One hundred and twenty healthy Kunming mice were randomly divided into 6 groups：the normal control group,sham operation group,14-day model group,28-day model group,14-day treatment group and 28-day treatment group.The model of cerebral ischemia reperfusion injury was constructed by griping bilateral carotid artery of mice for 15 minutes,and conducting reperfusion for another 15 minutes and repeating the above process 3 times. The treatment group was continuously given procyanidins by intragastric administration for 7 days before execution,and other groups were given the same amount of normal saline by intragastric administra-tion.The hippocampal tissue of mice was taken.ELISA was adopted to determine the expression of the tumor necrosis factor-α（TNF-α）and interleukin-6 （IL-6）and the expression of nuclear factor-κB （NF-κB）,and western blotting was adopted to observe the expression of NF-κB.Results：Compared with the model group,in each treatment group TNF-αand IL-6 protein expressions in mice brain tissue were decreased significantly （P 〈0.05）.NF-kappa B protein expressions of hippocampal tissues in treatment group were decreased significantly compared with the model group（P 〈0.05）.Conclusion：Procyandins can protect hippocampal neurons and reduce the inflammation in cerebral ischemia reper-fusion injury of mice,and the mechanism may be related to reduce the expression of NF-κB,TNF-αand IL-6.

关 键 词：脑缺血 再灌注损伤 原花青素 炎症 肿瘤坏死因子-α 白介素-6 核因子-ΚB 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R322.81[医药卫生—临床医学]