多聚赖氨酸与EDTA交联物对小鼠成纤维细胞的毒性研究  

Toxicity Study of Conjugates of PLL and EDTA on Mouse Fibroblast

作　　者：李英琦[1] 张海燕[1] 

机构地区：[1]贵阳医学院附院眼科,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第4期360-362,共3页Journal of Guiyang Medical College

基　　金：贵州省科技厅攻关项目[黔科合OZ字(20092)]

摘　　要：目的:观察多聚赖氨酸与乙二胺四乙酸(EDTA)的交联物(PLE)对体外培养的小鼠成纤维细胞L929的毒性程度。方法:小鼠成纤维细胞L929分为正常组、对照组、多聚赖氨酸组、PLE组和EDTA组,采用MTT法比较相同浓度下,EDTA、多聚赖氨酸与EDTA交联物对小鼠成纤维细胞L929相对增殖率的影响。结果:以正常组作为标准(100%),多聚赖氨酸组、EDTA组、PLE组和对照组对小鼠成纤维细胞L929的相对增殖度(RGR)分别为79.87%、69.35%、81.47%和20.12%,均较正常组下降,差异有统计学意义(P<0.05);在质量浓度达为1 000μmo L/L时,EDTA对小鼠成纤维细胞L929的毒性为2级,PLE和多聚赖氨酸对小鼠成纤维细胞L929的毒性为1级。结论:实验浓度的PLE和EDTA对小鼠成纤维细胞L929毒性较低,具有一定的安全性。Objective：To investigate the toxicity degree of PLE from EDTA and Poly-L-Lysine （PLL）on fibroblast L929 of mouse in vitro culture.Methods：Mouse fibroblast L929 were divided in-to normal group,control group,PLL group,PLE group and EDTA group,adopting MTT method to compare the effect of EDTA and PLE on proliferation of mouse fibroblast L929 under the same concen-tration.Results：Taking normal group as standard （100%）,relative growth rate （RGR）of L929 in PLL group,EDTA group,PLE group and control group were 79.87%、69.35%、81.47% and 20.12%,lowered than normal group,differences were statistically significant （P 〈0.05 ）.As the mass concentration reached 1 000 μmoL/L,toxicity of EDTA on fibroblast L929 reached level 2,PLE group reached level 1.Conclusions：Experiment concentration PLE and EDTA has minor toxicity on mouse fibroblast L929.

关 键 词：多聚赖氨酸 乙二胺四乙酸 成纤维细胞 细胞毒性 

分 类 号：R776.1[医药卫生—眼科]