2012年江苏省柯萨奇病毒A组14型分离株全基因序列分析  

Genetic characteristics of complete genome for Coxsakievirus A14 isolated in 2012,in Jiangsu Province

作　　者：姚昕[1] 卞莲莲[1] 毛群颖[1] 叶强[1] 朱凤才[2] 梁争论[1] 

机构地区：[1]中国食品药品检定研究院肝炎病毒疫苗室,北京100050 [2]江苏省疾病预防控制中心,江苏南京210000

出　　处：《微生物学免疫学进展》2015年第1期13-16,共4页Progress In Microbiology and Immunology

基　　金：科技部重大专项(2013ZX09304101)

摘　　要：目的探讨2012年江苏省手足口病(HFMD)病原体CVA14病毒的全基因序列特点。方法采集江苏省邳州市2012年HFMD患儿的咽拭子或肛拭子样本,分离培养得到2株CVA14病毒,利用RT-PCR扩增CVA14全长基因。利用DNAStar软件包的Meg Align进行核苷酸序列分析,构建系统进化树。结果扩增得到覆盖CVA14全基因组的3个片段,拼接后为CVA14全基因组,分别命名为PZ05Y/JS/2012和PZ15G/JS/2012。同源性分析结果显示,这2株分离株之间同源性为99.4%,原型株(G-14)的核苷酸同源性为84.4%。进化树结果显示,所有的CVA14形成A、B和C共3个分支。结论获得了江苏省CVA14的全基因序列,分析了该毒株的进化特点,补充了中国CVA14病毒的基因信息。Objective To investigate the characteritics for the complete genome of Coxsackievirus A14 （CVA14） from patients suffered from hand foot and mouth disease （HFMD） in 2012, in Jiangsu. Methods Throat swabs and rectal swabs were collected from children with HFMD. Two CVA14 strains were isolated and the complete genome was obtained by using RT-PCR. The nucleotide sequence was analyzed and phylogenetic trees were constructed by using MegAlign in DNAStar. Results Complete genome of CVA14 was obtained by splicing 3 PCR fragments. The two strains were named PZ05Y/JS/ 2012 and PZ15G/JS/2012, respectively. Sequence analysis showed that the two CVA14 strains shared 99.4% nucleotide homology, and shared 84.4% nucleotide homology with the prototype （ strain G-14）. Phylogenetic trees established from alignment of the complete genome and VP1 region showed that all CVA14 strains formed 3major clusters as A, B and C. Conclusion The characterization of the complete genome sequence and epidemiology of CVA14 were achieved from isolates in Jiangsu and their＇ s evolutive properties were analyzed. Our results will provide the new information for CVA14 sequence in China.

关 键 词：手足口病(HFMD) 柯萨奇病毒A组14型(CVA14) 基因型 

分 类 号：R373.23[医药卫生—病原生物学]