布拉酵母菌散对肝硬化大鼠肠黏膜屏障保护作用的实验研究  

The protective effect of Sacchromyces boulardii on intestinal mucosal barrier of rats with cirrhosis

作　　者：庞超[1] 胡义亭[2] 郑吉敏[2] 刘娜[2] 侯洪涛[2] 王玉珍[2] 

机构地区：[1]河北医科大学研究生学院,河北石家庄050011 [2]河北省人民医院消化科,河北石家庄050051

出　　处：《中国微生态学杂志》2015年第3期289-292,共4页Chinese Journal of Microecology

摘　　要：目的研究布拉酵母菌散对肝硬化大鼠肠黏膜屏障的保护作用及其可能机制。方法采用皮下注射40%CCL4橄榄油溶液+10%酒精饮料诱导肝硬化模型。造模成功后,治疗组用布拉酵母菌灌胃[75×108CFU/(kg·d)],对照组、模型组给予等容量的生理盐水灌胃,连续14 d。采用鲎试剂终点显色法检测血浆内毒素水平,应用分光光度法测定小肠组织二胺氧化酶(DAO)活性,应用自动生化分析仪检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)变化。结果模型组血浆内毒素水平[(0.251±0.011)EU/m L]较治疗组[(0.168±0.012)EU/m L]明显升高,差异有统计学意义(P<0.01);模型组肠组织DAO活性为(0.432±0.074)U/mg,明显较对照组[(0.728±0.065)U/mg]降低,差异有统计学意义(P<0.01),治疗组DAO活性为(0.548±0.053)U/mg,与模型组相比较,肠DAO活性升高,差异有统计学意义(P<0.01),模型组、治疗组肠组织DAO活性较对照组明显降低(P<0.01);模型组血清ALT、AST含量分别为(133.89±8.09)U/m L、(176.92±10.94)U/m L,比对照组ALT、AST含量[(36.73±6.95)U/m L、(41.35±10.07)U/m L]明显升高,P<0.01;治疗组[(95.76±7.27)U/m L、(158.02±5.94)U/m L]与模型组相比较,ALT、AST含量下降,差异有统计学意义(P<0.01)。结论肝硬化大鼠存在肠黏膜屏障受损,布拉酵母菌可通过调整肠道菌群,改善肠源性内毒素血症,对肠黏膜屏障起到保护作用,可作为慢性肝病的辅助治疗。Objective To investigate the protective effect and possible mechanism of Sacchromyces boulardii on intestinal mucosal barrier of rats with cirrhosis. Methods The rat models of cirrhosis were made by administering40% carbon tetrachloride and 10% ethanol solution. After 12 weeks,Sacchromyces boulardii was administered to rats in treatment group 75 × 10^8 CFU /（ kg·d） by gastric irrigation for 14 days,while the control group and model group were given equivalent dose of saline. The endpoint chromo-genic TAL assay was used to detect plasma endotoxin levels,spectrophotometry method to determine the activity of diamine oxidase（ DAO） in intestinal tissue,and automatic biochemical analyzer to detect serum ALT and AST levels. Results The concentration of endotoxin in the model group（ 0. 251 ± 0. 011） was significantly higher than that in the treatment group（ 0. 168 ± 0. 012）（ P〈0. 01）. The activity of DAO in the model group（ 0. 432 ± 0. 074） was significantly lower than that in the control group（ 0. 728 ± 0. 065）（ P〈0. 01）,and that in the treatment group（ 0. 548 ± 0. 053） was significantly higher than that in the model group（ P〈0. 01）; the difference between the treatment group and the control group was statistically significant（ P〈0. 01）. The concentrations of serum ALT and AST in model group（ 133. 89 ± 8. 09,176. 92 ± 10. 94） were significantly higher than those in the control group（ 36. 73 ± 6. 95,41. 35 ± 10. 07）（ P〈0. 01）,and those in treatment group were significantly lower than those in the model group（ P〈0. 01）. Conclusion The intestinal mucosa of rats with cirrhosis have a certain degree of injury; Sacchromyces boulardii can help to protect intestinal mucosal barrier from injury by cirrhosis through inhibiting bacterial translocation and intestinal endotoxemia,thus may be considered as an adjunctive therapy in the treatment of chronic liver disease.

关 键 词：肝硬化 布拉酵母菌 肠黏膜屏障 

分 类 号：R57[医药卫生—消化系统]