荸荠种质资源品种遗传多样性ISSR分析  

ISSR Analysis on Genetic Diversity of Water Chestnut( Eleocharis tuberosa Schulut) Cultivars

作　　者：江文[1,2] 陈丽娟[1] 欧昆鹏[1] 蔡炳华[1] 高美萍[1] 李杨瑞[2] 

机构地区：[1]广西农业科学院生物技术研究所,广西南宁530007 [2]广西农业科学院广西作物遗传改良生物技术重点开放实验室,广西南宁530007

出　　处：《西南农业学报》2015年第2期751-756,共6页Southwest China Journal of Agricultural Sciences

基　　金：广西科学研究与技术开发项目(桂科能14121008-1-12);国家科技支撑计划项目(2012BAD27B01);广西农业科学院发展基金项目(2013YM27;桂农科2012JZ04);广西农业科学院特色水生作物研究团队项目

摘　　要：为揭示不同荸荠栽培品种的遗传多样性,利用ISSR分子标记技术对24个荸荠品种的亲缘关系进行分析。结果表明,从100条ISSR引物中筛选出适合荸荠种质分析的11条ISSR引物,共扩增出86条带,平均每条引物扩增出7.8条带,多态性为69条带,多态性比例为80.3%。供试的24份荸荠品种两两之间的遗传距离值在0.0909-0.6071之间,平均值为0.3493。UPGMA法聚类结果表明24个荸荠品种在遗传距离0.34处可分为五类。24个荸荠品种在ISSR分析中亲缘关系较近,其遗传基础相对较狭窄,但部分不同地区栽培品种之间仍存在一定的遗传差异。The paper focused on assess the genetic variability and diversity of water chestnut（ Eleocharis tuberosa Schulut）. Genetic relation among 24 cultivars of water chestnut was analyzed by inter-simple sequence repeat（ ISSR） makers. 11 polymorphism primers were selected from 100 ISSR primers. A total of 86 ISSR bands were obtained,that is,7. 8 polymorphic ban each primer pair,and 69 of them were polymorphic,the polymorphism ratio was 80. 3 %. The dissimilarity coefficient of the 24 water chestnut cultivars ranged from 0. 0909 to 0. 6071,with an average of 0. 3493. The 24 water chestnut cultivars were divided into 5 groups at the genetic distance 0. 34 by UPGMA. The results showed that narrow genetic diversity and close genetic relationship presented among 24 water chestnut cultivars,and the difference varied in part of those cultivars from different areas,and the same as RAPD analysis at prophase.

关 键 词：荸荠 ISSR分子标记 聚类分析 遗传多样性 

分 类 号：S645.3[农业科学—蔬菜学]