3种文昌鱼的同工酶表型比较  

Comparison on Isozymic Phenotypes Among Three Amphioxus Species

作　　者：杨凤香[1] 胡闽[1] 房振华[1] 张秋金[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350117

出　　处：《福建师范大学学报（自然科学版）》2015年第3期83-87,共5页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家863计划资助项目(2008AA092602);福建省自然科学基金资助项目(2015J01126)

摘　　要：采用垂直平板不连续聚丙烯酰胺凝胶电泳和生化染色方法比较白氏文昌鱼(Branchiostoma belcheri)、日本文昌鱼(B.japonicum)和佛罗里达文昌鱼(B.floridae)的4种同工酶(苹果酸脱氢酶MDH、超氧化物歧化酶SOD、酯酶EST、淀粉酶α-AMY)的酶谱,结果显示4种同工酶酶带的数目、染色深浅及迁移率在3种文昌鱼之间的差异比较明显,可作为鉴定不同种文昌鱼的依据,但同种文昌鱼的雌雄之间以及性腺成熟前后的同工酶酶谱的差异不明显.A vertical fiat-bed discontinuous polyacrylamide gel electrophoresis technique was used to compare the phenotypes of four isozymes （ i. e. Malate dehydrogenase, Superoxide dis- mutase, Esterase and a-Amylase ） among three amphioxus species, Branchiostoma belcheri, B.japonicurn and B.floridae. It was found that there were significant differences in the number, staining intensity and relative mobility of enzymic spectrum among three species, and the differences of isozymic pattern can be used to distinguish the species. It can be also confirmed that B. belcheri and B. japoncurn are two separated species in Xiarnen waters in virtue of the difference of isozymic phenotypes between both species. For the same species, the electrophoretic patterns between female and male or between individuals with mature and immature gonad were slightly differences. It needs to be careful when the isozymic pattern was used to distinguish the sex.

关 键 词：文昌鱼 白氏文昌鱼 日本文昌鱼 佛罗里达文昌鱼 同工酶 

分 类 号：R151.3[医药卫生—营养与食品卫生学]