不同剂量1,25二羟基维生素预处理对局灶性脑缺血再灌注损伤的保护作用  

Protective effect of different dose of 1,25-dihydroxyvitamin preconditioningon focal cerebral ischemia reperfusion injury

作　　者：许峰峰[1] 刘信龙[1] 徐正平[1] 杨冰[1] 李明[1] 张建忠[1] 肖丽鸿[1] 廖环[1] 丁强[1] 李政卫 

机构地区：[1]解放军第455医院,上海200052

出　　处：《实用临床医药杂志》2015年第9期28-31,共4页Journal of Clinical Medicine in Practice

基　　金：全军医药卫生科研基金课题

摘　　要：目的探讨不同剂量1,25二羟基维生素预处理对局灶性脑缺血再灌注损伤的保护作用及机制。方法将60只SD雄性大鼠随机分为假手术组、模型组和VD30.5、1.0、1.5μg/kg预处理组,比较各组神经行为评分、炎性因子及TRL2、4水平。结果与模型组相比,随着VD3剂量的增加,神经行为评分和脑梗死面积均明显减少,VD3-3组改善最为明显(P<0.05);随着剂量的增高,各实验组IL-6和TNF-α水平降低越明显,具有明显的剂量变化趋势(P<0.05);IL-10、MDA各组变化差异无统计学意义(P>0.05);VD3各组TRL2和TRL4均明显低于模型组,且随着VD3剂量增加,TRL2和TRL4降低程度明显增加(P<0.05);再灌注5d后观察各组大鼠存活情况,以VD3-3组为最高83.33%,差异具有统计学意义(P<0.05)。结论 VD3对局灶性脑缺血再灌注损伤的保护作用具有剂量依赖性,其机制可能与抑制TLR2、4信号转导通路,减少炎性因子产生有关。Objective To investigate the protective effect and mechanism of different doses of 1,25-Dihydroxyvitamin preconditioning on focal cerebral ischemia reperfusion injury. Methods A total of 60 male SD rats were randomly divided into sham operation group,model group and VD3 pretreatment group with different dose of 0. 5,1. 0 and 1. 5 μg / kg. Neurobehavioral score,inflammatory factors and the level of TRL2,4 were compared among five groups. Results Compared with the model group,with the doses of VD3 increased,neurobehavioral score and area of cerebral infarction significantly decreased,VD3-3 group improved significantly（P〈 0. 05）. With the dose increased,levels of IL-6 and TNF-α decreased significantly,and the change showed obvious trend with dose（P〈 0. 05）. There were no significant differences of IL-10 and MDA among five groups（P〉 0. 05）.TRL2 and TRL4 in each VD3 group were significantly lower than those in the model group,and with the dose of VD3 increased,TRL2 and TRL4 decreased significantly（P〈 0. 05）. The survival conditions of rats in VD3 groups after 5 days of reperfusion were observed,8 3. 3 3 % in VD3-3 group was the highest,and there were significant differences（P〈 0. 05）. Conclusion The protective effect of VD3 on focal cerebral ischemia reperfusion injury is dose dependent,and its mechanism may be related to inhibition of signal transduction pathway of TLR2,4 and reduction of inflammatory factor production.

关 键 词：1 25二羟基维生素D3 局灶性脑缺血再灌注损伤 TLR2、4 炎性因子 

分 类 号：R743[医药卫生—神经病学与精神病学]