缺氧对结肠癌HT-29细胞Id1表达的影响  

Effect of inhibitor of differentiation-1 on expressions of colon carcinoma HT-29 cells under hypoxia environment in vitro

作　　者：于游 张才全[2] 

机构地区：[1]重庆市璧山区人民医院普外科,重庆400016 [2]重庆医科大学附属第一医院普外科,重庆400016

出　　处：《中国现代普通外科进展》2015年第3期178-181,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨分化抑制因子-1(Id1)在结肠癌细胞缺氧环境下的表达情况。方法:分别在体外1%O2缺氧条件下培养结肠癌HT-29细胞0h、6h、12h、24h及36h后,利用荧光定量聚合酶链反应(real-timePCR)及Westernblot方法检测各时点Id1的mRNA和蛋白表达情况。结果:缺氧能促进HT-29细胞Id1的mRNA和蛋白表达,缺氧12h后Id1的mRNA和蛋白表达至高峰。结论:在一定时限内,缺氧能促进结肠癌HT-29细胞Id1的mRNA和蛋白表达。Objective： To explore the Effect of inhibitor of differentiation-1 on expressions of colon carcinoma HT-29 cells under hypoxia environment in vitro. Method： The expressions of Id1 mRNA and protein were detected by fluorescence quantitative polymerase chain reaction（real-time PC R）and W estern blotassay in H T-29 cells under hypoxia 0h、6h、12h、24h、36h environmentin vitro respectively. Results： Hypoxia can promote the expressions of Id1 on mRNA and protein in colon cancer H T-29 cells. Conclusion： H ypoxia can promote the expressions of Id1 on mRNA and protein in colon cancer HT-29 cells.

关 键 词：分化抑制因子-1 结肠肿瘤 细胞增殖 荧光定量聚合酶链反应 

分 类 号：R735.35[医药卫生—肿瘤]