褪黑素对过氧化氢诱导晶状体上皮细胞氧化损伤的保护作用  

Study of melatonin on the protective effect of hydrogen peroxide-induced oxidative damage in human lens epithelial cells

作　　者：董天睿[1] 刘平[2] 倪锦红[1] 

机构地区：[1]哈尔滨医科大学附属第一医院妇科,中国黑龙江省哈尔滨市150001 [2]哈尔滨医科大学附属第一医院眼科,中国黑龙江省哈尔滨市150001

出　　处：《国际眼科杂志》2015年第5期764-766,共3页International Eye Science

基　　金：国家自然科学基金(No.30973275)~~

摘　　要：目的:探讨褪黑素对过氧化氢诱导晶状体上皮细胞氧化损伤的保护作用。方法:晶状体上皮细胞传代培养后,分别加入不同浓度褪黑素预处理12h后,加入100μmol/L H2O2继续孵育24h,MTT比色法检测褪黑素对H2O2诱导的晶状体上皮细胞活力的影响,流式细胞仪检测细胞凋亡率,比色法检测凋亡相关因子Caspase-3及Caspase-9的活性。结果:MTT结果显示褪黑素对晶状体上皮细胞活性无影响,该药物可以抑制过氧化氢诱导的细胞活性的下降,流式细胞计数结果显示褪黑素可以抑制过氧化氢诱导的细胞凋亡,此外,褪黑素还可以减少过氧化氢所致晶状体上皮细胞内Caspase-3及Caspase-9的活性,并且,伴随褪黑素作用时间的延长其活性呈下降趋势。结论:褪黑素可以明显抑制过氧化氢诱导的晶状体上皮细胞的凋亡,从而为寻求有效的防治白内障药物提供可靠的实验依据。AIM： To investigate theprotective effect of melatonin against hydrogen peroxide （ H2 O2 ）-induced oxidative damage to human lens epithelial cells. METHODS： Sub-culture human lens epithelial cells preprocessed with different concentrations of melatonin for 12h and then 100 μmol/L H2 O2 for 24h. The impact of melatonin on H2 O2-induced lens epithelial cell viability was detected by MTT assay, rate of apoptosis was detected by flow cytometry instrument and activity of apoptosis-related factors, Caspase-3 and Caspase-9, were detected by colorimetric method. RESULTS： MTT assay showed that melatonin had no effect on the activity of lens epithelial cells, and the drug can inhibit the decrease of H2 O2-induced cell activity, as well as flow cytometry showed that melatonin can inhibit H2 O2-induced apoptosis. ln addition, melatonin can also reduce H2 O2-induced Caspase-3 and Caspase-9 activity in lens epithelial cells, and their activity decreased with effect of melatonin along with extending time. CONCLUSION： Melatonin can obviously inhibit H2 O2 -induced apoptosis of human lens epithelial cells, which provide reliable experimental basis for drug on treatment of cataract.

关 键 词：褪黑素 晶状体上皮细胞 氧化损伤 

分 类 号：R776.1[医药卫生—眼科]