Structural characterization and biological activities of SC4, an acidic polysaccharide from Salvia chinensis  

石见穿酸性多糖SC4的结构特征和生物活性

作　　者：刘翠平[1] 方积年[1] 李晓玉 萧晓秋 

机构地区：[1]中国科学院上海生命科学研究院上海药物研究所,上海中国200031

出　　处：《Acta Pharmacologica Sinica》2002年第2期162-166,共5页中国药理学报（英文版）

摘　　要：To study the chemical characterization and some biological activities of an acidic polysaccharide, named SC4 from Salvia chinensis. METHODS: Chemical and spectral methods were employed to identify the structural characterization of SC4. Effect of SC4 on the proliferation of T- and B-lymphocytes both in vivo and in vitro was measured at various concentrations, and the protective effects of SC4 on PC12 cells against H2O2-induced injury was observed. RESULTS: SC4 was a highly branched polysaccharide with mean molecular weight of 4.5×105, composed of Rha, Xyl, Ser, Gal, and GalA in the molar ratio of 1.0: 7.0:5.3:1.2:4.2. Methylation analysis and NMR identified the linkages of the residues of SC4. SC4 increased the B-lymphocyte proliferation and spleen weight remarkably while no obvious changes were examined in T-lymphocyte proliferation, thymus weight, and the level of IgG and C3 in blood of mice. Moreover, SC4 enhanced the PC12 cells viability after H2O2 treatment. CONCLUSION: SC4, the acidic polysaccharide with complicated structure, was a B-lymphocyte stimulator and protected PC12 cells at the concentration of 20 mg/L against H2O2-induced injury.目的:研究从石见穿(Salvia chinensis)中得到的酸性多糖SC4的化学结构和生物活性.方法:利用化学和光谱方法分析了SC4的结构特征,并观察了SC4对淋巴细胞增殖的影响以及增强PC12细胞对抗H_2O_2造成的损伤的作用.结果:SC4的分子量为4.5×10~5,由Rha,Xyl,Ser,Gal和GalA组成,摩尔比为1.0:7.0:5.3:1.2:4.2.甲基化和^(13) C NMR进一步确定了SC4中各糖残基的连接方式.SC4能显著促进B淋巴细胞的增殖,增加小鼠脾重,增强PC12细胞对抗H_2O_2造成的氧化损伤的能力.结论:SC4为一多分枝的酸性多糖,其免疫活性的靶细胞为B淋巴细胞,并显著增强PC12细胞对抗H_2O_2造成的损伤的能力.

关 键 词：Salvia chinensis POLYSACCHARIDES molecular structure T-LYMPHOCYTES B-LYMPHOCYTES PC12 cells biomolecular nuclear magnetic resonance 

分 类 号：R284[医药卫生—中药学]