绿原酸分子印迹电化学传感器的制备及其性能分析  

Preparation and properties of electrochemical sensor for chlorogenic acid based on molecular imprinted polymers

作　　者：李利军[1] 张雷[1] 崔福海[1] 程昊[1] 李彦青[1] 

机构地区：[1]广西科技大学生物与化学工程系,柳州545006

出　　处：《分析试验室》2015年第5期503-506,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(310602191);广西高校科学技术研究项目(2013YB176);广西科技大学科学基金项目(校科自1261115)资助

摘　　要：应用分子印迹技术,以绿原酸为模板分子,邻苯二胺为功能单体,利用循环伏安法在玻碳电极表面构建了绿原酸的电化学传感器。采用差分脉冲伏安法,研究了此传感器的分析性能,建立了以K3[Fe（CN）6]为电子传递媒介的对绿原酸的间接分析方法。在8.0×10-7~2.0×10-5mol/L范围内,绿原酸的浓度与K3[Fe（CN）6]的相对峰电流变化呈较好的线性关系,检出限为1.4×10-7mol/L。将其用于实际样品清肝利胆口服液中绿原酸的测定,回收率为101.6%~105.5%。An electrochemical sensor based on melorcularly imprinted films for chlorogenic acid （ CGA ） detection is reported. The sensor was prepared by electropolemerizing o-phenylenediam-ine （OPD） on a glassy carbon electrode in the presence of template chlorogenic acid. Differential pulse voltammetry was applied to investigate the analytical characteristics of the sensor, and indirect analytical method based on K3 [ Fe（ CN）6] which is as electron transfer medium was established. The results indicate that the method has high selectivity and sensitivity, the relative peak current of sensor shows a linear dependance on the concentration of chlorogenic acid in the range of 8.0 × 10 -7 ~ 2. 0 × 10-7mol/L, with the detection limit of 1.4× 10-Tmol/L. This sensor was successfully applied to determination of chlorogenic acid in qinggan lidan oral liquid samples with recoveries ranging from 101.6% to 105.5%.

关 键 词：绿原酸 邻苯二胺 分子印迹 电化学传感器 

分 类 号：O657.1[理学—分析化学]